Immunoexpression of Type IV Collagen in the Intervertebral Disc

Chang Hoon Jeon; Ji-Ho Moon; Yong Ho Kang

doi:10.4184/jkss.2002.9.3.172

Journal List > J Korean Soc Spine Surg > v.9(3) > 1036012

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Jeon, Moon, and Kang: Immunoexpression of Type IV Collagen in the Intervertebral Disc

Original Article

Journal of Korean Spine Surg 2002;9(3):172-177.

Published online: 21 February 2002

DOI: https://doi.org/10.4184/jkss.2002.9.3.172

Immunoexpression of Type IV Collagen in the Intervertebral Disc

Chang Hoon Jeon, M.D., Ji-Ho Moon, M.D., Yong Ho Kang, M.D.^∗

Department of Orthopedic Surgery, Ajou University School of Medicine, Suwon, Korea

^∗Department of Orthopedic Surgery, Konyang University College of Medicine, Taejon, Korea

Address reprint requests to Chang-Hoon Jeon, M.D. Department of Orthopaedic Surgery, Ajou University School of Medicine San5, Wonchon-dong, Paldal-gu, Suwon 442-721, Korea Tel : 82-331-219-5220, Fax : 82-331-219-5229, E-mail : chjeon@ajou.ac.kr

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Study Design

In vitro studies using human intervertebral disc for the localization of the type IV collagen.

Objective

1) To study the distribution pattern and immunoexpression of type 4 collagen in the intervertebral disc, 2) To study the function of type IV collagen in the intervertebral disc.

Summary of Back Ground

The correlations of degeneration changes and collagens in the dics have not been determined. The reports for type IV collagen were few. So far, the histologic analysis for the expression of type IV collagen in the intervertebral disc has not been done. There was no report to study the function of the type IV collagen in the intervertebral disc.

Methods

Fifty- four disc blocks obtained during anterior interbody fusion of the lumbar spine were used to observe the expression pattern of the type IV collagen with immunochemical stain. For the observation of the myxomatous degeneration in the intervertebral disc, the alcian blue stain with periodic acid- schilff was done. For the control group, 22 neonate intervertebral disc blocks were obtained at autopsy.

Results

The immunoreactions for type IV collagen were associated blood vessels in the anulus fibrosus in the disc. There was no statistical significant difference of the type IV collagen expression between the control and disease groups. Myxomatous degenerations were observed as the irregular form in the degenerative intervertebral disc.

Conclusion

The immunoreactions for the type IV collagen were observed in the intervertebral discs and associated with the formation of the blood vessels, especially in anulus fibrosus.

Keywords: Intervertebral disc, Type IV collagen, Blood vessel

REFERENCES

1). Ahmed MU, Thorepe SR, Baynes JW. Identification of N (carboxymethyl)-lysine as a degradation product of fructose lysine in glycated protein. J Biol Chem. 261:4889–4894. 1986.

2). Beard HK, Ryvar R, Brown R, Muir H. Immunochemical localization of collagen types and proteoglycan in pig intervertebral discs. Immunology. 41:491–501. 1980.

3). Beard HK, Roberts S, O’ Brien JP. Immunofluores -cent staining for collagen and proteoglycan in normal and scoliotic intervertebral discs. J Bone Joint Surg. 63B:529–534. 1981.

4). Berkovitz B. Collagen crimping in the intra-articular disc and articular surfaces of the human temporo -mandibular joint. Arch Oral Biol. 45:749–756. 2000.

5). Brickley-Parsons D, Glimcher MJ. Is the chemistry of collagen in intervertebral discs an expression of Wolff's law? A study of the human lumbar spine. Spine. 9:148–163. 1984.

6). Eyre DR. Collagens of the disc. Chosh P, editor. ed.The Biology of the Intervertebral Disc. Boca Raton, Florida: CRC Press;p. 171–188. 1988.

7). Ghosh P, Bushelll GR, Taylor TFK, Akeson WH. Collagens, elastins and noncollagenous protein of the intervertebral disc. Clin Orthop. 129:124–132. 1977.

8). Herbert CM, Lindberg KA, Jayson MIV, et al. Changes in the collagen of human intervertebral discs during ageing and degenerative disc disease. J Mol Med. 1:79–91. 1975.

9). Kaapa E, Holm S, Han X, Takala T, Kovanen V, Vanharanta H. Collagens in the injured porcine intervertebral disc. J Orthop Res. 12:93–102. 1994.

10). Nerlich AG, Boos N, Wiest I, Aebi M. Immunolo -calization of major interstitial collagen types in human lumbar intervertebral discs of various ages. Vichows Archv. 432(1):67–76. 1998.

Figures and Tables%

Fig. 1.

The myxomatous degenerations(*) in the degenerative intervertebral disc were observed in irregular form (×200).

Fig. 2.

Extracellular matrix fibers were seen with regular form(*) in the neonate disc (× 200).

Fig. 3.

Blood vessels associated with the type IV collagen was abserved in anulus fibrosus (× 100).

Fig. 4.

Type IV Collagen was expressed with blood vessels in neonate disc (× 200).

Table 1.

The immunoexpression of type IV collagen.

	Type IV co	ollagen
	+	-
Neonate	22	0
Internal disc derangement	12	0
Isthmic spondylolisthesis	24	0
Spinal stenosis with instability	18	0

(P > 0.05, P* > 0.05)

Table 2.

The results of alcin-blue in the intervertebral disc.

	Myxomatous degeneration
	+	-
Neonate	0	22
Internal disc derangement	12	0
Isthmic spondylolisthesis	26	0
Spinal stenosis with instability	18	0

(P < 0.05)

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