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Abstract
Study Design
Immunohistologic staining of Intervertebral discs at surgery was performed using CD68, matrix metallopro-teinase- 1(MMP- 1) and matrix metalloproteinase- 3(MMP- 3).
Objectives
1. To investigate the histologic characteristics of the macrophage infiltrations in herniated discs and degenerative discs. 2. To investigate the difference of immumoexpression of MMP- 1 and MMP- 3 activity to herniated discs and degenerative discs. 3. To investigate the possible correlation between the macrophage infiltrations, MMP- 1and MMP- 3 in herniated discs and degenerative discs.
Summary of Literature Review
Spontaneous absorption of herniated disc is associated with infiltrating macrophages and matrix metalloproteinases. Matrix metalloproteinases(MMP) are main matrix degradative enzymes in intervertebral disc.
Materials and Methods
Sixty- seven specimens of degenerative disc specimens and one hundred and two specimens of lumbar disc herniation were immunostained with monoclonal antibodies to CD68, anti- MMP- 1, and anti- MMP- 3.
Results
The granulation tissue containing many CD68- positive macrophages was commonly observed in herniated disc and not in degenerative disc. The immunoexpression of MMP- 1 and MMP- 3 were observed in herniated and degenerative disc and there were the differences of intensity and localization of MMP- 1and MMP- 3 expression in herniated disc and not in degenerative disc. There was no correlation between the macrophage infiltrations, MMP- 1and MMP- 3 expression.
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