Detection Rate of Rapid Antigen Test for Pandemic Influenza A (H1N1 2009)

Young Keun Kim; Hyo Youl Kim; Young Uh; Jin-Kyong Chun

doi:10.3947/ic.2010.42.2.95

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Kim, Kim, Uh, and Chun: Detection Rate of Rapid Antigen Test for Pandemic Influenza A (H1N1 2009)

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Infection & Chemotherapy

Infection & Chemotherapy 2010; 42(2): 95-98.

Published online: 30 April 2010

DOI: https://doi.org/10.3947/ic.2010.42.2.95

Detection Rate of Rapid Antigen Test for Pandemic Influenza A (H1N1 2009)

Young Keun Kim¹, Hyo Youl Kim¹, Young Uh², Jin-Kyong Chun³

¹Department of Internal Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea.

²Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea.

³Department of Pediatrics, Yonsei University Wonju College of Medicine, Wonju, Korea.

Corresponding author: Hyo Youl Kim, M.D., Ph.D. Division of Infectious Diseases, Department of Internal Medicine, Yonsei University Wonju College of Medicine, 162, Ilsan-dong, Wonju, 220-701, Gngwon-do, Korea. Tel: +82-33-741-1206, Fax: +82-33-748-1206, hyksos@yonsei.ac.kr

Received 26 February 2010 Accepted 20 April 2010

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

There are few datas on the diagnostic accuracy of rapid antigen test for pandemic influenza A (H1N1). We evaluated the performance of rapid antigen test for the diagnosis of pandemic influenza A (H1N1). During the period from 21 August 2009 to 20 September 2009, 451 patients with influenza-like symptom underwent the rapid antigen test (SD Influenza Antigen, Standard Diagnostics, Yongin, Korea) and real-time reverse transcriptase-polymerase chain reaction (rRT-PCR) at the same time. Rapid antigen test results were positive for 65 of 90 patients with the positive results for pandemic influenza A (H1N1) on rRT-PCR assay. The sensitivity of the rapid antigen test was 72.2% (95% CI, 61.8-81.1) and the specificity was 100% (95% CI, 99.0-100). The positive predictive value was 100% (95% CI, 94.5-100) and negative predictive value was 93.5% (95% CI, 90.6-95.8).

Keywords: Influenza A, Pandemic, Reverse Transcriptase Polymerase Chain Reaction, Rapid Antigen Test, Sensitivity

Figures and Tables

Table 1

Performance of the Rrpid Influenza Aantigen Test for Diagnosis of Pandemic Influenza A (H1N1) according to Age

RIDT, rapid influenza diagnostic test; CI, confidence interval; PPV, positive predictive value; NPV, negative predictive value; N/A, not applicable

Table 2

Performance of the Rapid Influenza Antigen test for Diagnosis of Pandemic Influenza A (H1N1) according to Symptom Interval

RIDT, rapid influenza diagnostic test; CI, confidence interval; PPV, positive predictive value; NPV, negative predictive value; N/A, not applicable

Table 3

The Positivity of Rapid Antigen Test According to the Cycle Threshold (Ct) Value of Real-time RT-PCR

RIDT, rapid influenza diagnostic test

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