Soon-Ho Park; Yiel-Hea Seo; Jeong-Yeal Ahn; Pil-Hwan Park; Kyung-Hee Kim; Young-Hee Song; Jung-Eun Kim

doi:10.3947/ic.2010.42.1.35

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Park, Seo, Ahn, Park, Kim, Song, and Kim: Characteristics of CTX-M Type Extended Spectrum β-lactamase Producing Non-typhoi dal Salmonella Isolates

Original Article

Infection & Chemotherapy

Infection & Chemotherapy 2010; 42(1): 35-38.

Published online: 28 February 2010

DOI: https://doi.org/10.3947/ic.2010.42.1.35

Characteristics of CTX-M Type Extended Spectrum β-lactamase Producing Non-typhoi dal Salmonella Isolates

Soon-Ho Park, Yiel-Hea Seo, Jeong-Yeal Ahn, Pil-Hwan Park, Kyung-Hee Kim, Young-Hee Song, Jung-Eun Kim

Department of Laboratory Medicine, Gachon University Gil Hospital, Incheon, Korea.

Corresponding Author: Yiel-Hea Seo, M.D. Department of Laboratory Medicine, Gachon University Gil Hospital, 1198 Guwol-dong, Namdong-gu, Incheon, 405-760, Korea. Tel: +82-32-460-3074, Fax: +82-32-460-3415, seoyh@gilhospital.com

Received 7 December 2009 Accepted 9 January 2010

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Extended-spectrum β-lactamase (ESBL)-producing Salmonella have been increasingly reported worldwide. ESBL-producing Salmonella is of particular concern since children cannot be treated with quinolones. This study was conducted to determine the phenotypic and genetic characteristics of ESBL-producing Salmonella in a tertiary hospital.

Materials and Methods

Four clinical ESBL-producing isolates of non-typhoidal Salmonella were collected during 2001 to 2009. Antimicrobial susceptibility was determined by disk diffusion test and VITEK-II system. ESBL production was tested by ESBL phenotypic confirmatory test. TEM, SHV, CTX-M1, CTX-M2, CTX-M8, and CTX-M9 type ESBL genes were detected by PCR amplification, and PCR products were subjected to direct sequencing.

Results

Phenotypic confirmatory test showed that 4 of the 300 non-typhoidal Salmonella isolates were ESBL-producing: 3 S. Enteritidis and 1 S. Typhimurium. All 4 isolates were recovered during the past 1 year period. All 3 S. Enteritidis harbored CTX-M-15, while the S. Typhimurium harbored CTX-M-14. All CTX-M-15-producing S. Enteritidis isolates showed resistance both to cefotaxime and ceftazidime, while the CTX-M-14-producing S. Enteritidis were resistant only to cefotaxime.

Conclusions

ESBL-producing nontyphoidal Salmonella has emerged recently and the type of ESBL has switched from TEM and SHV to CTX-M.

Keywords: Legionella pneumophila, Diagnosis, Biomarker, cDNA microarray

Figures and Tables

Table 1

Clinical features of the patients infected with ESBL-producing non-typhoidal Salmonella

M, male; st, stool; bl, blood; ur, urine

Table 2

Minimal inhibitory concentrations (µg/mL) of ESBL producing non-typhoidal Salmonella isolates by Vitek-II AST-N056 card

AM, ampicillin; ATM, aztreonam; FEP, cefepime; CTX, cefotaxime; CTT, cefotetan; IPM, imipenem; CAZ, ceftazidime; CIP, ciprofloxacin; PIP, piperacillin; PIP/TAZ, piperacillin/tazobactam; SXT, trimethoprim/sulfamethoxazole

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