A Case of Arcanobacterium haemolyticum Bacteremia and Osteomyelitis Diagnosed by 16s rRNA Sequencing

Soo Young Bae; Soomi Choi; Seung-Ji Kang; Hee-Chang Jang; Kyung Hwa Park; Sook-In Jung; Jong-Hee Shin

doi:10.3947/ic.2010.42.4.241

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Bae, Choi, Kang, Jang, Park, Jung, and Shin: A Case of Arcanobacterium haemolyticum Bacteremia and Osteomyelitis Diagnosed by 16s rRNA Sequencing

Case Report

Infection & Chemotherapy

Infection & Chemotherapy 2010; 42(4): 241-243.

Published online: 30 August 2010

DOI: https://doi.org/10.3947/ic.2010.42.4.241

A Case of Arcanobacterium haemolyticum Bacteremia and Osteomyelitis Diagnosed by 16s rRNA Sequencing

Soo Young Bae¹, Soomi Choi¹, Seung-Ji Kang¹, Hee-Chang Jang¹, Kyung Hwa Park¹, Sook-In Jung¹, Jong-Hee Shin²

¹Department of Internal Medicine, Chonnam National University Medical School, Gwangju, Korea.

²Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea.

Correspondence to Hee-Chang Jang, M.D. Department of Internal Medicine, Chonnam National University Hospital, 671, Jebong-ro, Dong-gu, Gwangju 501-757, Korea. Tel: +82-62-220-6296, Fax: +82-62-225-8578, haroc153@naver.com

Received 13 November 2009 Revised 13 January 2010 Accepted 24 March 2010

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Arcanobacterium haemolyticum is a catalase-negative, aerobic gram-positive rod. It causes pharyngitis, skin and soft tissue infection, osteomyelitis, endocarditis, meningitis, pneumonia, and septicemia. We experienced a case of A. haemolyticum bacteremia and osteomyelitis in a diabetic patient. The organism was misidentified as Cellulomonas species by automated system but correctly identified as A. haemolyticum by 16s rRNA sequencing.

Keywords: Arcanobacterium, Bacteremia, Sequence analysis, RNA

Figures and Tables

Figure 1

Findings of osteomyelitis in tri-phase bone scan. Bony uptake of left 1st proximal and distal phalanges is not observed in delayed phase image. Increased uptakes of left 1st metatarsal bone head, tarsal bone, and upper portion of calcaneous are observed in delayed phase image.

Figure 2

Reverse CAMP test. Arcanobacterium haemolyticum is streaked horizontally and Staphylococcus aureus ATCC 26923 was streaked vertically. The inhibition zone of hemolysis of Staphylococcus ATCC 25923 is noted near the streak line of Arcanobacterium haemolyticum.

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