Woo-Sung Lee; Seong-Kyun Kim; Seong-Joo Heo; Jai-Young Koak; Joo-Hee Lee; Ji-Man Park; Yoon-Kyung Park

doi:10.4047/jkap.2014.52.4.298

Journal List > J Korean Acad Prosthodont > v.52(4) > 1034796

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Lee, Kim, Heo, Koak, Lee, Park, and Park: Osseointegration of the titanium implant coated with rhTGF-β2/PLGA particles by electrospray: a preliminary microCT analyzing rabbit study

Original Article

J Korean Acad Prosthodont 2014;52(4):298-304.

Published online: 9 February 2014

DOI: https://doi.org/10.4047/jkap.2014.52.4.298

Osseointegration of the titanium implant coated with rhTGF-β2/PLGA particles by electrospray: a preliminary microCT analyzing rabbit study

Woo-Sung Lee¹, Seong-Kyun Kim^1,^∗, Seong-Joo Heo¹, Jai-Young Koak¹, Joo-Hee Lee², Ji-Man Park³, Yoon-Kyung Park⁴

¹Department of Prosthodontics, Seoul National University Dental Hospital, School of Dentistry, Seoul National University, Seoul, Republic of Korea

²Department of Prosthodontics, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Republic of Korea

³Department of Prosthodontics, Ewha Womans University, Seoul, Republic of Korea

⁴Department of Dental Research Institute, Brain Korea 21, Seoul National University, Seoul, Republic of Korea

^∗Corresponding Author: Seong-Kyun Kim Department of Prosthodontics, Seoul National University Dental Hospital, School of Dentistry, Seoul National University, 101 Daehak-ro, Jongno-gu, Seoul, 110-744, Republic of Korea +82 2 2072 2661: e-mail, ksy0617@snu.ac.kr

^∗ This study was supported by a grant (2011-434) from the Asan Institute for Life Sciences, Seoul, Korea and National Research Foundation of Korea grant funded by the Korea government (No. 2011-0028067), and by a grant of the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (A120304).

Received 5 August 20145 September 2014 Accepted 16 September 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

This preliminary rabbit study was conducted to evaluate the effect of recombinant human transforming growth factor-β 2 (rhTGF-β 2)/poly lactic-co-glycolic acid (PLGA) coating on osseointegration of the titanium (Ti) implant.

Materials and methods

Eight Ti implants were anodized with 300 voltages for three minutes. Four of those were coated with rhTGF-β 2/PLGA by an electrospray method as the experimental group. The implants were placed into tibiae of four New Zealand rabbits, two implants per a tibia, one implant per each group. After 3 and 6 weeks, every two rabbits were sacrificed and micro-computed tomography (microCT) was taken for histomorphometric analysis.

Results

In scanning electron microscope (SEM) image, the surface of rhTGF-β 2/PLGA coated Ti implant showed well distributed particles. Although statistically insignif-icant, microCT analysis showed that experimental group has higher bone volume / total volume (BV/TV) and trabecular thickness (Tb.Th) values relatively. Cross sectional view also showed more newly formed bone in the experimental group.

Conclusion

In the limitation of this study, rhTGF-β 2/PLGA particles coating on the Ti implant show the possibility of more favorable quantity of newly formed bone after implant installation.

Keywords: TGF-beta2, PLGA, Titanium, Implant, Osseointegration

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Fig. 1.

Schematic image of implant. (A) Region of interest (ROI) for microCT,(B) reference for cross sectional view.

Fig. 2.

SEM image of implant surface (×5,000). (A) Control group (implant without surface coating), (B) Experimental group (implant with rhTGF-β 2/PLGA coating via electrospray method).

Fig. 3.

MicroCT analysis. (A) BV/TV (bone volume per total volume), (B) SMI (structure model index), (C) Tb.Th (trabecular thickness), (D) Tb.N (trabecular number), C (control), E (experimental) (P>.05).

Fig. 4.

Cross sectional view of microCT. (A) 3 weeks control, (B) 3 weeks experimental (rhTGF-β 2/ PLGA coated implant), (C) 6 weeks control, (D) 6 weeks experimental (rhTGF-β 2/ PLGA coated implant).

Table 1.

MicroCT analysis, mean (SD)

Parameter	3w		6w
Parameter	Control	Experimental	Control	Experimental
TV (mm³)	25.520 (4.750)	23.401 (11.201)	25.447 (5.557)	20.401 (8.887)
BV (mm³)	2.978 (1.161)	3.267 (0.458)	5.029 (2.829)	5.345 (2.444)
BV/TV (%)	12.304 (6.838)	15.237 (5.334)	19.000 (6.967)	26.061 (0.629)
SMI	2.157 (0.691)	1.813 (0.826)	1.377 (0.621)	1.601 (0.209)
Tb.Th (mm)	0.037 (0.006)	0.044 (0.001)	0.055 (0.009)	0.086 (0.010)
Tb. N (1/mm)	3.245 (1.319)	3.516 (1.344)	3.406 (0.734)	3.052 (0.265)

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