A study of mesenchymal stem cell proliferation and surface characteristics of the titanium discs coated with MS275/PLGA by an electrospray

Soo-Yeon Yoo; Seong-Kyun Kim; Seong-Joo Heo; Jai-Young Koak; Joo-Hee Lee; Yoon-Kyung Park; Ena Kim

doi:10.4047/jkap.2012.50.4.285

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Yoo, Kim, Heo, Koak, Lee, Park, and Kim: A study of mesenchymal stem cell proliferation and surface characteristics of the titanium discs coated with MS275/PLGA by an electrospray

Original Article

The Journal of Korean Academy of Prosthodontics

The Journal of Korean Academy of Prosthodontics 2012; 50(4): 285-291.

Published online: 30 October 2012

DOI: https://doi.org/10.4047/jkap.2012.50.4.285

A study of mesenchymal stem cell proliferation and surface characteristics of the titanium discs coated with MS275/PLGA by an electrospray

Soo-Yeon Yoo, DDS, MSD¹, Seong-Kyun Kim, PhD¹, Seong-Joo Heo, PhD¹, Jai-Young Koak, PhD¹, Joo-Hee Lee, PhD², Yoon-Kyung Park, PhD¹, Ena Kim, BSD¹

¹Department of Prosthodontics, Seoul National University Dental Hospital, School of Dentistry, Seoul National University, Seoul, Korea.

²Department of Prosthodontics, Seoul Asan Medical Center, Ulsan University, Seoul, Korea.

Corresponding Author: Seong-Kyun Kim. Department of Prosthodontics, Seoul National University Dental Hospital, School of Dentistry, Seoul National University, 28 Yeongum-Dong, Chongno-Gu, Seoul, 110-749, Korea. +82 2 2072 2661: ksy0617@snu.ac.kr

Received 17 September 2012 Revised 9 October 2012 Accepted 16 October 2012

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

This study was conducted to identify the surface characteristics of titanium discs coated with MS275/PLGA by electrospray and which is effective to mesenchymal stem cell proliferation.

Materials and methods

We used anodized surface coated with PLGA as a control group and anodized surface coated with MS275 0.5 µM, 1 µM, 1.5 µM as test groups. To examine that the coating particles are nanometer sized, FE-SEM was used and AFM was utilized to determine the difference of coating surface roughness. We checked the mesenchymal stem cell proliferation by using MTT assay on 1^st, 4^th, 7^th days.

Results

There was no significant difference between control groups and test groups in AFM results (P>.05). In MTT assay results, mesenchymal stem cell proliferation was increased with time, at 7^th day, cell viability on discs coated with 1.5 µM MS275 was significantly higher than control group (P<.05). As SEM showed, the number of cells on all discs was increased and the morphology of cell attachment was also wider and closer with time.

Conclusion

Titanium surface coated with MS275/PLGA showed significantly higher cell proliferation and the more density of MS275 was dispersed on titanium discs, the faster cells grew.

Keywords: Titanium, MS275, PLGA, Mesenchymal stem cells

Figures and Tables

Fig. 1

SEM (×5000) image of anodized titanium disc by electrospraying (A) PLGA + DMSO (control)/disc. Anodized titanium discs are coated with submicron-sized PLGA particles. (B) 0.5 µM MS275 (25 µl) + PLGA (25 µl))/disc. Anodized titanium disc coated with PLGA/MS275 shows more sticky and fine texture than control group. (C) 1.0 µM MS275 (25 µl) + PLGA (25 µl))/disc (d) 1.5 µM MS275 (25 µl) + PLGA (25 µl))/disc.

Fig. 2

MTT assay measured as cell proliferation. The result shows that cell proliferation is increased by time and after 7 day of cell culture, there is a significant difference (^*P<.05; the graph bar represents mean ± SE). And the cell activity of test groups (anodized titanium disc coated with PLGA + MS275 0.5 µM, 1.0 µM, 1.5 µM) is significantly higher than control group (anodized titanium disc coated with PLGA/DMSO) at 7 day.

Fig. 3

SEM (×500) images of 1 day cell culture (A) PLGA + DMSO (control) /disc, (B) 0.5 µM MS275 (25 µl) + PLGA (25 µl))/disc, (C) 1.0 µM MS275 (25 µl) + PLGA (25 µl))/disc, (D) 1.5 µM MS275 (25 µl) + PLGA (25 µl))/disc, SEM (×500) images of 4 day cell culture, (E) PLGA + DMSO (control) /disc, (F) 0.5 µM MS275 (25 µl) + PLGA (25 µl))/disc, (G) 1.0 µM MS275 (25 µl) + PLGA (25 µl))/disc, (H) 1.5 µM MS275 (25 µl) + PLGA (25 µl))/disc, SEM (×500) images of 7 day cell culture, (I) PLGA + DMSO (control)/disc, (J) 0.5 µM MS275 (25 µl) + PLGA (25 µl))/disc, (K) 1.0 µM MS275 (25 µl) + PLGA (25 µl))/disc, (L) 1.5 µM MS275 (25 µl) + PLGA (25 µl))/disc.

Fig. 4

The results of surface roughness measured by AFM (20 µm × 20 µm) (A) Anodized titanium disc coated with PLGA, (B) Anodized titanium disc coated with MS275 and PLGA. There is no significant difference between group (A) and (B) (P>.05).

Table 1

Surface roughness (Ra) of anodized disc coated with PLGA (control group) and MS275 + PLGA (test group)

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