Journal List > J Korean Acad Prosthodont > v.50(2) > 1034693

Oh, Seo, Ahn, Park, Kang, and Song: Surface roughness and Candida albicans adhesion to flexible denture base according to various polishing methods

Abstract

Purpose

The purpose of this study was to compare the effect of 3 chairside polishing methods and laboratory polishing methods on surface roughness and C. albicans adhesion of polyamide denture base.

Materials and methods

Using contact profilometer, the surface of polyamide specimens (25×15×2 mm) was studied after conventional polishing without finishing and after chiarside polishing with 2 chiarside polishing kits and chairside-pumice polishing following finishing with tungsten carbide bur. To evaluate the adhesion of C. albicans, C. albicans suspension was overlayed on the test specimen. And the specimens were incubated for 2 hours. Imprint culture method was achieved and counted the colony on the agar plate. Polished polyamide were evaluated using a scanning electron microscope. The statistics were conducted using one-way ANOVA and in case of difference, Scheffe test and Tamhane's T2 test were used.

Results

Surface roughness (Ra) of surfaces polished with 2 chairside polishing kits had higher than conventional polishing and pumice polishing. The highest roughness value was 0.32 ± 0.10 µm, and the lowest was 0.02 ± 0.00 µm. The adhesion of C. albicans on the specimens polished with chairside polishing group and pumice polishing group were increased than conventional polishing group (P<.01).

Conclusion

Conventional laboratory polishing was found to produce the smoothest surface and the lowest adhesion of C. albicans. Two groups polished with Chairside polishing kits were similar with respect to surface roughness. Surface of the specimen polished with pumice is significantly smoother than 2 chairside polishing groups, but the result of C. albicans adhesion is that group polished with pumice was similar with 2 chairside polishing groups (P>.01).

Figures and Tables

Fig. 1
Profilometer.
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Fig. 2
Surface roughness test.
*means significant difference at P<.01 statistically.
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Fig. 3
SEM image of polished polyamide surfaces. Control group had regular surface. Scratch of SP group was tighter than S group.
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Fig. 4
The colonies of C. albicans on the agar plate.
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Fig. 5
The growth of C. albicans on the agar plate.
*means significant difference at P<.01 statistically.
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Fig. 6
Comparison of results in this study.
*and dotted line mean significantly difference at P<.01 statistically.
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Table 1
Experimental materials used in this study
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Jae-Min Seo
https://orcid.org/http://orcid.org/0000-0001-5095-4046

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