The effect of different crystallization temperature of the hydroxyapatite coating produced by ion beam-assisted deposition on anodizing-treated titanium disks on human osteosarcoma cells

Ahran Pae; Hyun-Du Won; Richard Leesungbok; Hyeong-Seob Kim; Yi-Hyung Woo

doi:10.4047/jkap.2011.49.4.333

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This article has been corrected. See "Erratum: The effect of different crystallization temperature of the hydroxyapatite coating produced by ion beam-assisted deposition on anodizing-treated titanium disks on human osteosarcoma cells" in Volume 50 on page 216.

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Pae, Won, Leesungbok, Kim, and Woo: The effect of different crystallization temperature of the hydroxyapatite coating produced by ion beam-assisted deposition on anodizing-treated titanium disks on human osteosarcoma cells

ORIGINAL ARTICLE

J Korean Acad Prosthodont 2011;49(4):333-340.

Published online: 18 December 2011

DOI: https://doi.org/10.4047/jkap.2011.49.4.333

The effect of different crystallization temperature of the hydroxyapatite coating produced by ion beam-assisted deposition on anodizing-treated titanium disks on human osteosarcoma cells

Ahran Pae, DMD, MSD, PhD, Hyun-Du Won, DMD, MSD, PhD, Richard Leesungbok, DMD, MSD, PhD, Hyeong-Seob Kim, DMD, MSD, PhD, Yi-Hyung Woo, DMD, MSD, PhD^∗

Department of Prosthodontics, School of Dentistry, Kyung Hee University, Seoul, Korea

∗Corresponding Author: Yi-Hyung Woo Department of Prosthodontics, School of Dentistry, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, Korea +82 2 958 9340: e-mail, yhwoo@khu.ac.kr

Received 12 October 201120 October 2011 Accepted 24 October 2011

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

The aim of this study was to study the effect of hydroxyapatite (HA) coating crystallinity on the proliferation and differentiation of human osteosarcoma cells.

Materials and methods

Surface roughness of the titanium disks increased by anodizing treatment and then HA was coated using ion beam-assisted deposition (IBAD). HA coating was crystallized by heat-treated at different temperature (100℃, 300℃, 500℃, 800℃). According to the temperature, disks were divided into four groups (HA100, HA300, HA500, HA800). With the temperature, crystallinity of the HA coating was different. Anodized disks were used as control group. The physical properties of the disk surface were evaluated by surface roughness tests, XRD tests and SEM. The effect of the crystallinity of HA coating on HOS cells was studied in proliferation and differentiation. HOS cells were cultured on the disks and evaluated after 1, 3, 5, and 7 days. Growth and differentiation kinetics were subsequently investigated by evaluating cell proliferation and alkaline phosphatase activity.

Results

Regardless of the heat-treated temperature, there is no difference on the surface roughness. Crystallinity of the HA was appeared in the groups of HA500, HA800. HOS cells proliferation, ALP activity were higher in HA500 and HA800 group than HA100 and HA300.

Conclusion

Within the results of this limited study, heat treatment at 500℃ of HA coating produced by IBAD has shown greater effect on proliferation and differentiation of HOS cells. It is considered that further in vivo study will be necessary. (J Korean Acad Prosthodont 2011;49:333-40)

Keywords: Cell proliferation, Alkaline phophatase activity

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Fig. 1.

SEM morphologies (×2,000). A: anodizing treated (control group), B: HA100, C: HA300, D: HA500, E: HA800 group shows some cracks (indicated arrows).

Fig. 2.

The results of X-ray diffraction (XRD) test. At HA500 and HA800 group, HA peak appeared but there is no appearance of HA peak at HA100 and HA300 group. A: control group, B: HA100, C: HA300, D: HA500, E: HA800.

Table 1.

Classification of the test groups

Name	Treatment conditions
Control	Anodizing only
HA100	Anodizing + IBAD + Heat treatment at 100℃
HA300	Anodizing + IBAD + Heat treatment at 300℃
HA500	Anodizing + IBAD + Heat treatment at 500℃
HA800	Anodizing + IBAD + Heat treatment at 800℃

Table 2.

The results of surface roughness tests

	Control	HA100	HA300	HA500	HA800
Ra (μ m)	0.36	0.34	0.37	0.37	0.30
Ry (μ m)	2.62	2.57	2.26	2.46	2.28
Rz (μ m)	2.07	1.90	1.91	2.03	1.72
Sm (μ m)	0.069	0.068	0.076	0.06	0.044
s (μ m)	0.018	0.019	0.018	0.018	0.016

Ra = Average roughness, Ry = Rmax (maximum height roughness), Rz = 10 points median height, Sm = Spacing of surface peaks, s = Mean spacing of local peaks of the profile

Table 3.

Mean (O.D 570) of the MTT assay results

	1 day		3 day		5 day		7 day
	Mean	SD	Mean	SD	Mean	SD	Mean	SD
Control	0.144^a	0.005	0.442^c,d	0.012	1.103	0.014	1.282	0.011
HA100	0.141^a	0.003	0.418^c	0.014	1.099	0.032	1.274	0.010
HA300	0.140^a	0.006	0.458^c,d,e	0.015	1.098	0.034	1.278	0.016
HA500	0.166^b	0.002	0.471^d,e	0.008	1.105	0.036	1.292	0.040
HA800	0.168^b	0.001	0.487^d,e	0.019	1.107	0.034	1.287	0.014

∗Scheffe's test for variable (P<.05)

Groups with the same letter are not significantly different.

Table 4.

Mean (O.D 405) of the ALP assay results

	1 day		3 day		5 day		7 day
	Mean	SD	Mean	SD	Mean	SD	Mean	SD
Control	0.181^a,b,c	0.002	0.387	0.015	0.535	0.025	0.792	0.017
HA100	0.177^a,b	0.005	0.381	0.061	0.468	0.004	0.576^e	0.013
HA300	0.192^c	0.006	0.431	0.061	0.466^d	0.023	0.616^e	0.056
HA500	0.185^b,c	0.004	0.418	0.009	0.592	0.020	1.040^f	0.021
HA800	0.169^a	0.004	0.435	0.021	0.668	0.014	1.014	0.078

∗Scheffe's test for variable (P<.05)

Groups with the same letter are not significantly different.

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