Jung-Bo Huh; Dong-Hee Jung; Ji-Sun Kim; Sang-Wan Shin

doi:10.4047/jkap.2010.48.4.259

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Huh, Jung, Kim, and Shin: Effects of different sizes of Hydroxyapatite/β-Tricalcium phosphate particles on vertical bone augmentation

Original Article

The Journal of Korean Academy of Prosthodontics

The Journal of Korean Academy of Prosthodontics 2010; 48(4): 259-265.

Published online: 31 October 2010

DOI: https://doi.org/10.4047/jkap.2010.48.4.259

Effects of different sizes of Hydroxyapatite/β-Tricalcium phosphate particles on vertical bone augmentation

Jung-Bo Huh, DDS, MSD, Dong-Hee Jung, DDS, MSD, Ji-Sun Kim, DDS, MSD, Sang-Wan Shin, DDS, PhD

Advanced Prosthodontics, Graduate School of Clinical Dentistry, Korea University / Institute for Clinical Dental Research, Seoul, Korea.

Corresponding Author: Sang-Wan Shin. Advanced prosthodontics, Graduate School of Clinical Dentistry, Korea University/Institute for Clinical Dental Research, 97 Guro-dong, Guro-gu, Seoul, 152-703, Korea. +82 2 2626 1922: swshin@korea.ac.kr

Received 22 July 2010 Revised 30 August 2010 Accepted 11 October 2010

Abstract

Purpose

This study was aimed to evaluate the effect of different sizes of β-TCP/HA particles on vertical bone augmentation using titanium mesh in the cranium of rabbits.

Material and methods

Six white rabbits weighing 5kg were used. Four circular grooves of 6mm diameter were made by trephine, and five small holes were drilled in the inner surface of each circular gooves. Different sizes of grafts (small 0.3 - 0.5 mm, medium 0.5 - 1.0, large 1.0 - 2.0 mm) were placed respectively in the experimental groups. Titanium mesh (height 3 mm, width 6 mm) was placed. After 8weeks healing period, the rabbits were euthanized, and the specimens were prepared for histological findings. New bone formation and remaining graft area were measured to calculate the ratio of areas occupying the inner space of titanium mesh. Mann-Whitney U-test and Wilcoxon signed rank-test were used for statistical analysis (α = .05).

Results

The experimental groups with β-TCP/HA graft showed a significantly higher new bone formation (P = .003). Comparing different sizes of β-TCP/HA, there was no statistical difference in terms of new bone formation. The vertical bone formation (i.e. new bone and graft area) was significantly greater in β-TCP/HA groups (P = .001). In comparison between different sizes of β-TCP/HA, medium size group had significantly greater area than large particle size group (P = .039).

Conclusion

The use of β-TCP/HA with titanium mesh showed a higher vertical bone formation, particularly the medium sized β-TCP/HA particles (0.5 - 1.0 mm) produced better results in vertical bone augmentation.

Keywords: β-TCP/HA, Titanium mesh, Particle size, Vertical augmentation

Figures and Tables

Fig. 1

A: A flap was made using a midsagittal incision and exfoliated from the forehead. The periosteum was incised and lifted to expose the cranium on both sides of the midline, B: A circular groove was prepared on each side of the midline using a trephine drill. Five small holes were drilled with 1mm diameter round bur to induce bleeding from the bone marrow space within the circle.

Fig. 2

A: The experimental titanium mesh caps were filled with β-TCP/HA as the experimental site or without β-TCP as the control site, B: Fixation of meshes including β-TCP/HP by titanium pins.

Fig. 3

Sagittal histological section of the titanium mesh (×12.5).

A : control group, no β-TCP/HA, newly generated tissue reaches to almost the height of the titanium mesh and there is a little newly formed bone. B (large particle size), C (medium particle size), D (small particle size): experimental groups, containing β-TCP/HA, Newly generated tissue, newly formed bone has formed surrounding β-TCP/HA. β-TCP/HAP particles remain in the upper part of the titanium mesh. there is more newly formed bone than in control group. In large particle size, there are less β-TCP/HA particles than other experimental groups.

Fig. 4

High magnification of the section of the titanium mesh containing medium sized β-TCP/HA (×40).

newly formed bone (○), β-TCP/HA (△), newly generalized ground tissue (□).

nuclear of osteocyte (↓)

Nuclear: black, Erythrocyte: orange, Cytoplasm: red, Collagen: green.

Fig. 5

Comparison of percentage areas of New bone and β-TCP/HA.

Table 1

Percentage areas of New bone and β-TCP/HA

Values are mean±SD (%)

^*P = .003 ^**P = .002 ^***P = .04

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