Abstract
Statements of the problem
Many denture wearers occasionally use denture adhesives to improve denture retention, stability and chewing efficiency. An ideal denture adhesive is nontoxic, non-irritating, and provides comfort to the oral mucosa.
Purpose
The purpose of this study was to evaluate the cytotoxicity and adhesive properties of a selected denture adhesive.
Material and methods
To test cytotoxicity of the selected denture adhesive, mouse fibroblast cells were used in MTT testing. Cytotoxicity was examined according to the concentration of the denture adhesive and incubated for 1 to 4 days. To examine adhesive property, a denture base was fabricated on an edentulous dentiform. The adhesive was applied to the denture base, then tensile bond strength was measured, to evaluate the change in retention during 3 days.
Results andConclusion
1. 1% and 2% concentration denture adhesive cream had no cytotoxicity. 2. The tensile bond strength of the group with both denture adhesive and artificial saliva was significantly higher than that of the group with only denture adhesive (P < .05). The tensile bond strength of the group with denture adhesive was significantly higher than that of with only artificial saliva (P < .05). 3. The tensile bond strength had no significant change during 1 hour, and then gradually decreased. After 1 day, it decrease to half. Within the limitation of this study, the tested denture adhesive had no cytotoxicilty and was effective in improving denture retention. The adhesive strength began to continuously decrease after 1 hour and it decreased to half at 1 day after application.
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Table I.
Table II.
Component | Content |
---|---|
distilled water | 2000 ml |
gastric mucin | 4.4 g |
NaCl | 0.762 g |
CaCl2 2H2O | 0.426 g |
KH2PO4 | 1.476 g |
KCl | 2.228 g |
pH | 6.8 |
Table III.
Cell proliferation | Cytotoxicity grade |
---|---|
60 - 100 | Not cytotoxic |
30 - 60 | Moderate cytotoxic |
0 - 30 | Severe cytotoxic |
Table IV.
1 day | 2 days | 3 days | 4 days | |
---|---|---|---|---|
control | 0.82 (0.10) | 1.51 (0.23) | 2.03 (0.16) | 2.44 (0.20) |
1% | 0.64 (0.07) | 1.25 (0.25) | 1.48 (0.22) | 1.70 (0.23) |
2% | 0.70 (0.10) | 1.21 (0.19) | 1.58 (0.33) | 1.71 (0.30) |
Table V.
P | 1 day | 2 days | 3 days | 4 days | Cytotoxicity |
---|---|---|---|---|---|
Control | 100.00 | 100.00 | 100.00 | 100.00 | Not |
1% | 77.84 | 82.93 | 72.82 | 69.80 | Not |
2% | 85.06 | 80.33 | 77.68 | 70.11 | Not |
Cytotoxicity | Not | Not | Not | Not |