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Abstract
STATEMENT OF PROBLEM
A biochemical approach for surface modification has offered an alternative for physicochemical and morphological methods to obtain desirable bone-implant interfaces.
PURPOSE
The purpose of the present study was to investigate cell responses to poly (D,L-lactide-co-glycolide) (PLGA)/1α, 25-(OH)2D3 coating with reference to cellular proliferation and differentiation in vitro.
MATERIAL AND METHODS
96 titanium discs were fabricated and divided into four groups. Group 1 was anodized under 300 V as control. Group 2, 3 and 4 were anodized then coated with 3 ml PLGA/1α, 25-(OH)2D3 solutions. Amount of the solutions were 2 ul, 20 ul and 200ul respectively. The osteoblast-like Human Osteogenic Sarcoma (HOS) cells were seeded and cultured for 1, 3 and 7 days. MTS-based cell proliferation assay and ALPase activity test were carried out.
RESULTS
PLGA nanoparticles were observed as fine, smooth and round and HOS cells attached to the anodized surfaces through strand-like and sheet-like filopodia. After 3 days of culture, the dendritic filopodia were exaggerated and sheet-like cytoplasmic projections covered the coated titanium surfaces. After 3 days of culture, all of the groups showed increased cellular proliferation and the lowest proliferation rate was measured on group 2. Higher amount of incorporated 1 α, 25-(OH)2D3 (Group 3 and 4) improved cellular proliferation but the differences were not significant statistically (P > .05). But they increased the rate of ALP activities than the control group at day 3 (P < .05).
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Fig. 1.
SEM surface morphology of titanium disc surfaces (×1000) after 1-day culture (a) Group 1: Anodized under 300 V (b) Group 2: Anodized under 300 V then, coated with 3 ml [PLGA/1α, 25-(OH)2D3 (2 ul/disc)] solution (c) Group 3: Anodized under 300 V then, coated with 3 ml [PLGA/1α, 25-(OH)2D3 (20 ul/disc)] solution (d) Group 4: Anodized under 300 V then, coated with 3 ml [PLGA/1α, 25-(OH)2D3 (200 ul/disc)] solution
Fig. 2.
SEM surface morphology of titanium disc surfaces (×1000) after 3-day culture (a) Group 1: Anodized under 300 V (b) Group 2: Anodized under 300 V then, coated with 3 ml [PLGA/1α, 25-(OH)2D3 (2 ul/disc)] solution (c) Group 3: Anodized under 300 V then, coated with 3 ml [PLGA/1α, 25-(OH)2D3 (20 ul/disc)] solution (d) Group 4: Anodized under 300 V then, coated with 3 ml [PLGA/1α, 25-(OH)2D3 (200 ul/disc)] solution
Fig. 3.
SEM surface morphology of titanium disc surfaces (×1000) after 7-day culture (a) Group 1: Anodized under 300 V (b) Group 2: Anodized under 300 V then, coated with 3 ml [PLGA/1α, 25-(OH)2D3 (2 ul/disc)] solution (c) Group 3: Anodized under 300 V then, coated with 3 ml [PLGA/1α, 25-(OH)2D3 (20 ul/disc)] solution (d) Group 4: Anodized under 300 V then, coated with 3 ml [PLGA/1α, 25-(OH)2D3 (200 ul/disc)] solution
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