Journal List > J Korean Soc Transplant > v.29(2) > 1034478

J Korean Soc Transplant. 2015 Jun;29(2):54-60. Korean.
Published online June 30, 2015.  https://doi.org/10.4285/jkstn.2015.29.2.54
Copyright © 2015 The Korean Society for Transplantation
Luminex-based Immunoassay for Organ Transplantation
Hyeyoung Lee, M.D. and Eun-Jee Oh, M.D.
Department of Laboratory Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Corresponding author: Eun-Jee Oh. Department of Laboratory Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 137-701, Korea. Tel: 82-2-2258-1641, Fax: 82-2-2258-1719, Email: ejoh@catholic.ac.kr
Received June 19, 2015; Revised June 20, 2015; Accepted June 20, 2015.

Abstract

Development of luminex-based solid phase assays enables advanced measurement of HLA antibody with sensitivity, specificity, and increasing knowledge of unacceptable antigens. In this review, we described the principle of the luminex-based assay and its current applications for organ transplantation including C1q assay, calculated panel reactive antibody, and virtual cross-matching. We also discussed the technical aspects and limitations for clinical utilization. The variables related to measurement of HLA antibody specificities and their clinical relevance remain unclear, therefore the interpretation of results requires comprehensive knowledge and clinical information in critical cases.

Keywords: Transplantation; Luminex; HLA antibody; Multiplex bead immunoassay

Tables


Table 1
Characteristics of three beads for luminex-based HLA antibody tests
Click for larger image

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