Study on Causes of Respiratory Disease to the Middle East Respiratory Syndrome-negative Subjects

Su-Jeong Hwang; Dong-Ju Park; Hee-Soo Koo; Ho-Cheol Yun; Pyeung-Tae Gu; Mi-Ok Lee; Sung-Hyun Jin

doi:10.4167/jbv.2017.47.3.156

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Hwang, Park, Koo, Yun, Gu, Lee, and Jin: Study on Causes of Respiratory Disease to the Middle East Respiratory Syndrome-negative Subjects

Original Article

Journal of Bacteriology and Virology 2017; 47(3): 156-164.

Published online: 30 September 2017

DOI: https://doi.org/10.4167/jbv.2017.47.3.156

Study on Causes of Respiratory Disease to the Middle East Respiratory Syndrome-negative Subjects

Su-Jeong Hwang, Dong-Ju Park, Hee-Soo Koo, Ho-Cheol Yun, Pyeung-Tae Gu, Mi-Ok Lee, Sung-Hyun Jin

Busan Metropolitan City Institute of Health & Environment, Busan, Korea.

Corresponding author: Su-Jeong Hwang. Busan Metropolitan City Institute of Health & Environment, 120, Hambakbong-ro 140 beon-gil, Buk-gu, Busan 46616, Korea. Phone: +82-51-309-2815, Fax: +82-51-309-2819, kies98@korea.kr

Received 3 July 2017 Revised 1 September 2017 Accepted 14 September 2017

The Korean Society for Microbiology and The Korean Society of Virology

(open-access):

This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/).

Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe cases of human respiratory disease. The current outbreak of infection with this virus in South Korea, which began on May 20, 2015, has infected 186 patients and caused 36 deaths within 2 months. In this study, to investigate the viral pathogen causing acute respiratory infections, multiplex/RT-PCR was performed on were obtained from nucleic acid of the Middle East Respiratory Syndrome-negative subjects. Viruses and atypical bacteria were detected in 39 of 337 (11.6%). Frequent viruses were human rhinovirus (n=11, 3.3%), human metapneumovirus (n=9, 2.7%), parainfluenza (n=9, 2.7%) and adenovirus (n=4, 1.2%). Mycoplasma pneumonia (M. pneumonia) was detected in 1.8 % (n=6). Out of 9 human metapneumovirus (hMPV) positive samples, 6 samples were successfully sequenced using F gene. And M. pneumoniae was sequencing of a repetitive region of the P1 gene. Phylogenetic analysis revealed that hMPV clustered into A2b lineage (n=4), B2 lineage (n=2) and M. pneumoniae clustered into two genotypes: Type 1 (n=4), Type 2a (n=2).

Keywords: Middle East respiratory syndrome coronavirus (MERS-CoV), Human Metapneumovirus (hMPV), Mycoplasma pneumonia (M. pneumonia), Phylogenetic analysis

Figures and Tables

Figure 1

Age and sex distribution of respiratory pathogens. Percentage of positive samples for each age group: 19~29 y, 3.5%; 30~39 y, 14.0%; 40~49 y, 14.3%, 50~59 y, 25.0%; ≥60 y, 9.2%.

Figure 2

Phylogenetic analysis for HMPV based on of the F protein. The nucleotide sequences were aligned using Clustal W. Trees using the neighbor-joining method with Mega 4. The scale bars show the proportions of nucleotide substitutions, and the numbers at the branches are bootstrap values determined for 1,000 iterations. Only bootstrap values with ≥70% significance are shown.

Table 1

Primers used in this study

Table 2

Detection status of respiratory pathogens

ADV: adenovirus, hBoV: human bocavirus, PIV:parainfluenza virus, RSV: respiratory syncytial virus, IFV: influenza virus, hCoV: human coronavirus, hRV: human rhinovirus, hMPV: human Metapneumovirus, Mp: Mycoplasma pneumonia, Cp: Chlamydophila pneumoniae, Bp: Bordetella pertussis

Table 3

Respiratory pathogens finding according to patient's age groups

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