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Kim, Park, and Hwang: Prevalence and Molecular Characteristics of Methicillin-resistant Staphylococcus aureus Isolates in a Neonatal Intensive Care Unit
Communication

J Bacteriol Virol 2016;46(2):99-103.

Published online: 9 February 2016

DOI: https://doi.org/10.4167/jbv.2016.46.2.99

Prevalence and Molecular Characteristics of Methicillin-resistant Staphylococcus aureus Isolates in a Neonatal Intensive Care Unit

Sun Young Kim, So Hae Park, Soo Myung Hwang∗

Department of Clinical Laboratory Science, Catholic University of Pusan, Korea

∗Corresponding author: Soo Myung Hwang. Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan, Busan 46252, Korea. Phone: +82-51-510-0563, Fax: +82-51-510-0568, e-mail: smhwang@cup.ac.kr

Received 9 May 201615 June 2016       Accepted 21 June 2016

Copyright © 2016 Journal of Bacteriology and Virology

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) isolated from neonates in a neonatal intensive care unit (NICU) were investigated by multilocus sequence typing (MLST), staphylocoagulase (SC) genotyping, staphylococcal cassette chromosome mec (SCC mec) typing, accessory gene regulator (agr) typing, and the presence of Panton-Valentine leukocidin (PVL). Among the 44 S. aureus isolates from nares in neonates between March and June 2014 at hospital in Busan, 27 (61.4%) were MRSA and 17 (38.6%) were methicillin-susceptible S. aureus (MSSA). The most prevalent clone in MRSA isolates was ST72-SC type Vb-SCC mec IV-agr I (n=26) and the remaining one was ST89-SC type I-SCC mec II-agr II. In MSSA isolates, the prevalent clone was ST121-SC type Va-agr IV (n=13), followed by ST72-SC type Vb-agr I (n=2), ST8-SC type III-agr I (n=1) and ST15-SC type X-agr II (n=1). All isolates did not possess the PVL. The data showed that the neonates in NICU carried high prevalence of ST72 MRSA and remarkably different clones with SC diversity between MRSA and MSSA isolates.

Keywords: MRSA, NICU, Multilocus sequence type, Staphylocoagulase type, SCC mec type

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Table 1.
Molecular characteristics and antimicrobial susceptibility of MRSA and MSSA isolates
ST SC type No. of isolate SCC mec type agr type PVL No (%) of isolate susceptible to
OXA CL EM GM FA CIP MUP TET VA
ST72 Vb 26 IV I – 0 (0) 19 (73) 19 (73) 15 (0) 26 (100) 26 (100) 18 (69) 23 (88) 26 (100)
ST89 I 1 II II – 0 (0) 1 (100) 0 (0) 0 (0) 1 (100) 0 (0) 1 (100) 1 (100) 1 (100)
ST121 Va 13 MSSA IV – 13 (100) 13 (100) 13 (100) 6 (46) 1 (8) 13 (100) 2 (15) 13 (100) 13 (100)
ST72 Vb 2 MSSA I – 2 (100) 2 (100) 2 (100) 0 (0) 0 (0) 2 (100) 1 (50) 2 (100) 2 (100)
ST8 III 1 MSSA I – 1 (100) 1 (100) 1 (100) 1 (100) 1 (100) 1 (100) 0 (0) 1 (100) 1 (100)
ST15 X 1 MSSA II – 1 (100) 1 (100) 1 (100) 1 (100) 1 (100) 1 (100) 1 (100) 1 (100) 1 (100)

MRSA, methicillin-resistant Staphylococcus aureus; MSSA, methicillin-susceptible Staphylococcus aureus; ST, sequence type; SC, staphylocoagulase; SCC mec, staphylococcal cassette chromosome mec; OXA, oxacillin; CL, clindamycin; EM, erythromycin; GM, genta-mycin; FA, fucidic acid; CIP, ciprofloxacin; MUP, mupirocin; TET, tetracycline; VA, vancomycin.

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