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Park and Hwang: Emergence of Panton-Valentine Leukocidin-Positive ST80 Clone of Community-Associated Methicillin-Resistant Staphylococcus aureus in Busan, Korea
Communication

J Bacteriol Virol 2016;46(4):269-274.

Published online: 9 February 2016

DOI: https://doi.org/10.4167/jbv.2016.46.4.269

Emergence of Panton-Valentine Leukocidin-Positive ST80 Clone of Community-Associated Methicillin-Resistant Staphylococcus aureus in Busan, Korea

So Hae Park, Soo Myung Hwang∗

Department of Clinical Laboratory Science, Catholic University of Pusan, Busan, Korea

∗Corresponding author: Soo Myung Hwang. Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan, Busan 46252, Korea. Phone: +82-51-510-0563, Fax: +82-51-510-0568, e-mail: smhwang@cup.ac.kr

Received 4 November 201615 November 2016       Accepted 22 November 2016

Copyright © 2016 Journal of Bacteriology and Virology

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Community-associated methicillin resistant Staphylococcus aureus (CA-MRSA) has become widespread in the community and healthcare settings, and a number of clonal lineages emerged on every country. Sequence type (ST) 80 clone of CA-MRSA was dominant in Europe and has increasingly been isolated from the Middle East but so far never found in Korea. In this study, 48 MRSA isolates recovered from ear infections were characterized by multilocus sequence typing (MLST), staphylococcal cassette chromosome mec (SCC mec) typing, staphylocoagulase (SC) genotyping, staphylococcal protein A gene (spa) typing, accessory gene regulator (agr) typing, and virulence gene profiling. Most MRSA strains belonged to three major clones: ST5-SCC mec II-SC type II (n=19, 39.6%), ST239-SCC mec III-SC type IV (n=15, 31.2%), and ST72-SCC mec IV-SC type Vb (n=11, 22.9%). Among the isolates, one strain was Panton-Valentine leukocidin (PVL)-positive ST80-SCC mec IV-SC type XIa – spa type t044-agr group III, and exfoliative toxin D-positive. This strain was susceptible to most antibiotics, but resistant to tetracycline and fusidic acid. This is the first report on the emergence of European ST80 CA-MRSA clone in Korea.

Keywords: MRSA, ST80 clone, Multilocus sequence type, Staphylocoagulase type

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Figure 1.
Phylogenetic relationship among the nucleotide sequences of the D1 coa regions of the selected 9 isolates and 7 reference strains. The numbers at nodes indicate the bootstrap values based on 1000 replicates. ∗, reference strains; SC, staphylocoagulase type; Rep, a number of C-terminal repeat units of coa; spa, staphylococcal protein A gene; ST, sequence type.
jbv-46-269f1.tif
Table 1.
Molecular characteristics of 48 MRSA isolates from ear infections
MLSTa (strain) No. of isolates SCC mec type SCb type spa type (n) SSRc profile agr type Toxin genes se, tst, et (n) PVL
se, tst, et (n)
ST5 19 II II t002(17) t548(2) 26-23-17-34-17-20-17-12-17-16 26-23-17-34-17-20-17-12-16 II sec ∙ tst (14) tst (5) -
ST239 15 III IV t037(14) t633(1) 15-12-16-02-25-17-24 08-24 I sea (6) -
ST72 11 IV Vb t324(6) t664(5) 07-23-12-12-17-20-17-12-12-17 07-23-12-12-17-20-17-12-17 I - -
ST80 (SHD2) 1 IV XIa t044 07-23-12-34-34-33-34 III etd +
ST89 1 II I t375 49-13-23-05-17-34-33-34 III etb -
ST1 1 IV VII t286 07-23-13-34-16-34-33-13 III - -

a MLST, multilocus sequence type;

b SC, staphylocoagulase;

c SSR, short sequence repeats

Table 2.
Antimicrobial resistance rate of 48 MRSA isolates from ear infections
MLST No. of isolates % resistance
CLI CIP ERY FUS GEN TET TEI SXT VAN
ST5 19 100 100 95 16 68 89 0 0 0
ST239 15 100 93 100 20 100 87 0 0 0
ST72 11 27 0 82 0 18 0 0 0 0
ST80 1 0 0 0 100 0 100 0 0 0
ST89 1 0 0 100 0 100 0 0 0 0
ST1 1 0 0 100 0 100 0 0 0 0

CLI, clindamycin; CIP, ciprofloxacin; ERY, erythromycin; FUS, fusidic acid; GEN, gentamycin; TET, tetracycline; TEI, teicoplanin; SXT, trimethoprim-sulfamethoxazole; VAN, vancomycin.

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