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Abstract
Human respiratory syncytial virus (HRSV) is known as the leading cause of respiratory tract illness in infancy and elderly children worldwide. We investigate the prevalence pattern and genetic characteristics in the second variable region G protein gene of HRSV during 5 consecutive seasons from 2010 to 2015. A total of 4,793 specimens (throat swabs) were collected from patients with acute respiratory tract. HRSV were evaluated and classified as HRSV A (n=111) or HRSV B (n=64) by real-time RT-PCR or RT-PCR. In general HRSV were detected in winter season. Coughing, fever, rhinorrhea and sputum were confirmed main symptoms in patients with HRSV. There were no significant differences in clinical characteristics or severity according to the HRSV subgroup infections. Out of 175 HRSV positive samples, 94 samples were successfully sequenced using G gene. Phylogenetic analysis revealed that 62 HRSV-A strains clustered into genotypes ON1 (n=54, 87.1%), NA1 (n=7), NA2 (n=1) and 32 HRSV-B strains clustered into three genotypes: BA4 (n=28, 87.5%), BA5 (n=2), BA6 (n=2). These results provide a better understanding of HRSV prevalence pattern and genetic characteristics.
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Figure 3.
Phylogenetic trees for HRSV-A (A) and HRSV-B (B) based on the second variable region of the G protein. The nucleotide sequences were aligned using Clustal W. Trees using the neighbor-joining method with Mega 4. The scale bars show the proportions of nucleotide substitutions, and the numbers at the branches are bootstrap values determined for 500 iterations. Only bootstrap values with
Figure 4.
(A) Nucleotide characteristics of ON1 genotype and (B) nucleotide characteristics of BA genotype.
Table 1.
Detection of RSV from 2011/2012 to 2014/2015 seasons
| Season | No. of tested | No. of total positive | Positive rate (%) | No. of positive (%)∗ | |
|---|---|---|---|---|---|
| RSV A | RSV B | ||||
| 2010/2011 | 1,142 | 17 | 1.5 | 1 (5.9) | 16 (94.1) |
| 2011/2012 | 1,120 | 51 | 4.6 | 44 (86.3) | 7 (13.7) |
| 2012/2013 | 1,105 | 50 | 4.5 | 46 (92.0) | 4 (8.00) |
| 2013/2014 | 813 | 38 | 4.8 | 1 (2.6) | 37 (97.4) |
| 2014/2015 | 613 | 19 | 3.1 | 19 (100.0) | 0 (0.0) |
Table 2.
HRSV genotype distributions from 2011/2012 to 2014/2015 seasons
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