Journal List > J Bacteriol Virol > v.45(2) > 1034166

TOOLS
Kim, Seo, Park, Cho, Ha, Ha, Kim, Kang, Kim, Kim, and Chung: Molecular Epidemiology of Mumps Virus Circulated in Gwangju
Original Article

J Bacteriol Virol 2015;45(2):132-137.

Published online: 9 February 2015

DOI: https://doi.org/10.4167/jbv.2015.45.2.132

Molecular Epidemiology of Mumps Virus Circulated in Gwangju

Sun Hee Kim1, Mihee Seo1, Jung Wook Park1, Sun Ju Cho1, Yi Deun Ha1, Dong-Ryong Ha1, Eun-Sun Kim1, Hae Ji Kang2, Sung Soon Kim2, Kisoon Kim2, Jae Keun Chung1, ∗

1Health and Environment Institute of Gwangju, Gwangju, Korea

2National Institute of Health, Korea Centers for Disease Control and Prevection, Chungcheongbuk-do, Korea

∗Corresponding author: Jae Keun Chung. Health and Environment Institute of Gwangju, 149 Hwajeong-ro, Seo-gu, Gwangju, 502-837, Korea. Phone: +82-62-613-7630, Fax: +82-63-613-7549, e-mail: jkchung@korea.kr

∗∗ This research was performed as part of the laboratory investigation of legal infectious disease which is managed by Health & Environment Institute of Gwangju Metropolitan city.

Received 29 April 201528 May 2015       Accepted 2 June 2015

Copyright © 2015 Journal of Bacteriology and Virology

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The recent mumps epidemic in South Korea has generated a large amount of public concern. This study has attempted to analyze molecular epidemiological changes of mumps virus circulating in Gwangju metropolitan area, South Korea. 953 throat swab samples were collected from patients with parotitis from May 2013 to July 2014. The majority (71.5%) of these cases have occurred in middle or high school students aged from 15 to 19 years. All samples were tested using a reverse transcription polymerase chain reaction (RT-PCR) that targets the short hydrophobic (SH) gene of the virus. Mumps virus SH gene was detected in 39.2% (374/953) of samples. And 82 RT-PCR products were randomly selected for nucleotide sequencing analysis. All of these sequences were determined as genotype I by phylogenetic analysis and showed the highest nucleic acid similarity (99%) with Dg1062/Korea/98 (GenBank accession no. AY309060). These results suggested that appearance of new genotype or genetic variation at the nucleotide level could be ruled out to evaluate main cause of recent mumps outbreak in Gwangju metropolitan area.

Keywords: Mumps, Parotitis, Short hydrophobic gene, Genotype I

REFERENCES

1). Steven AR, Christian JS, Kathryn MC. Mumps Virus. Davis MK, Peter MH, editors. Fields Virology. 6th ed.Philadelphia: Lippincott Williams & Wilkins;2013. p. 1024–41.
2). Jin L, Örvell C, Myers R, Rota PA, Nakayama T, Forcic D, et al. Genomic diversity of mumps virus and global distribution of the 12 genotypes. Rev Med Virol. 2015; 25:85–101.
crossref
3). Korea Centers for Disease Control and Prevention. Disease web statistics system. Available from:. http://is.cdc.go.kr/nstat/index.jsp.
4). Barskey AE, Glasser JW, LeBaron CW. Mumps resurgences in the United States: A historical perspective on unexpected elements. Vaccine. 2009; 27:6186–95.
crossref
5). Kaaijk P, Van der Zeijst B, Boog M, Hoitink C. Increased mumps incidence in the Netherlands: review on the possible role of vaccine strain and genotype. Euro Surveill. 2008; 13.
crossref
6). Sane J, Gouma S, Koopmans M, de Melker H, Swaan C, van Binnendijk R, et al. Epidemic of mumps among vaccinated persons, the Netherlands, 2009–2012. Emerg Infect Dis. 2014; 20:643–8.
crossref
7). Park DW, Nam MH, Kim JY, Kim HJ, Sohn JW, Cho Y, et al. Mumps outbreak in a highly vaccinated school population: Assessment of secondary vaccine failure using IgG avidity measurements. Vaccine. 2007; 25:4665–70.
crossref
8). Ryu JU, Kim EK, Youn YS, Rhim JW, Lee KY. Outbreaks of mumps: an observational study over two decades in a single hospital in Korea. Korean J Pediatr. 2014; 57:396–402.
crossref
9). Okajima K, Iseki K, Koyano S, Kato A, Azuma H. Virological analysis of a regional mumps outbreak in the northern island of Japan-mumps virus genotyping and clinical description. Jpn J Infect Dis. 2013; 66:561–3.
10). Davidkin I, Jokinen S, Paananen A, Leinikki P, Peltola H. Etiology of mumps-like illnesses in children and adolescents vaccinated for measles, mumps, and rubella. J Infect Dis. 2005; 191:719–23.
crossref
11). Barskey AE1, Juieng P, Whitaker BL, Erdman DD, Oberste MS, Chern SW, et al. Viruses detected among sporadic cases of parotitis, United States, 2009–2011. J Infect Dis. 2013; 208:1979–86.
crossref
12). Palacios G, Jabado O, Cisterna D, de Ory F, Renwick N, Echevarria JE, et al. Molecular identification of mumps virus genotypes from clinical samples: standardized method of analysis. J Clin Microbiol. 2005; 43:1869–78.
crossref
13). Lee JY, Kim YY, Shin GC, Na BK, Lee JS, Lee HD, et al. Molecular characterization of two genotypes of mumps virus circulated in Korea during 1998–2001. Virus Res. 2003; 97:111–6.
14). Lee JY, Na BK, Kim JH, Lee JS, Park JW, Shin GC, et al. Regional outbreak of mumps due to genotype H in Korea in 1999. J Med Virol. 2004; 73:85–90.
crossref
15). Lee JY, Na BK, Lee HD, Chang SW, Kim KA, Kim JH, et al. Complete nucleotide sequence of a mumps virus Genotype I strain isolated in Korea. Virus Genes. 2004; 28:201–5.
crossref
16). Kashiwagi Y, Takami T, Mori T, Nakayama T. Sequence analysis of F, SH, and HN genes among mumps virus strains in Japan. Arch Virol. 1999; 144:593–9.
crossref
17). Vandermeulen C, Roelants M, Vermoere M, Roseeuw K, Goubau P, Hoppenbrouwers K. Outbreak of mumps in a vaccinated child population: a question of vaccine failure? Vaccine. 2004; 22:2713–6.
crossref
18). Davidkin I, Jokinen S, Broman M, Leinikki P, Peltola H. Persistence of measles, mumps, and rubella antibodies in an MMR-vaccinated cohort: a 20-year follow-up. J Infect Dis. 2008; 197:950–6.

Figure 1.
Number of mumps suspected cases and RT-PCR positive cases by monthly in an outbreak in Gwangju city, May 2013-July 2014 (n=953).
jbv-45-132f1.tif
Figure 2.
Number of mumps RT-PCR positive cases by age and sex in an outbreak in Gwangju city, May 2013-July 2014 (n=374).
jbv-45-132f2.tif
Figure 3.
Phylogenetic tree of selected mumps virus strains in the study and in GenBank. The tree is based on SH gene sequence alignment (206 bp). Strains detected in the study are marked with full dots (●). Capital letters denote mumps virus genotype.
jbv-45-132f3.tif
Table 1.
Results of RT-PCR using the mumps SH gene stratified by age group
Age in years Total <5 5~9 10~14 15~19 20~29 ≥30
No. of tested cases 953 58 98 77 681 19 20
No. of RT-PCR positive cases 374 4 5 25 326 8 6
RT-PCR positive rate (%) 39.2 6.9 5.1 32.5 47.9 42.1 30.0
TOOLS
Similar articles