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Yang, Nah, Kim, Choi, An, Kim, Park, and Song: Seroepidemiological Survey of Aujeszky's Disease Virus in Wild Boar (Sus scrofa) and Raccoon Dogs (Nyctereutes procyonoides koreensis) in Korea
Original Article

J Bacteriol Virol 2014;44(4):336-341.

Published online: 9 February 2014

DOI: https://doi.org/10.4167/jbv.2014.44.4.336

Seroepidemiological Survey of Aujeszky's Disease Virus in Wild Boar (Sus scrofa) and Raccoon Dogs (Nyctereutes procyonoides koreensis) in Korea

Dong-Kun Yang1, *, Jin-Ju Nah1, Ha-Hyun Kim1, Sung-Suk Choi1, Dong-Jun An1, Jong-Taek Kim2, Kyoung-Ae Park3, Jae-Young Song1

1Viral Disease Division, Animal and Plant Quarantine Agency, MAFRA, Anyang, Korea

2College of Veterinary Medicine, Kangwon National University, Korea

3Wild Life Center, Gyeonggi-do Veterinary Service Laboratory, Korea

*Corresponding author: Dong-Kun Yang. Animal and Plant Quarantine Agency, 175 Anyang-ro, Anyang-si, Gyeonggi-do 430-757, Korea. Phone: +82-31-467-1783, Fax: +82-31-467-1797, e-mail: yangdk@korea.kr

** This work was supported financially by a grant (F-1543083-2013-13-01) from Animal, and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs (MAFRA), Republic of Korea.

Received 1 August 201431 October 2014       Accepted 10 November 2014

Copyright © 2014 Journal of Bacteriology and Virology

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aujeszky's disease caused by Aujeszky's disease virus (ADV) is one of the most important diseases in the pig industry. In this study, we conducted a seroepidemiological survey of ADV in wild boars and raccoon dogs in South Korea. In total, 217 wild boar sera collected between March and August 2013, and 96 raccoon dogs between 2011 and 2012 were screened for the presence of antibodies against ADV. The sero-positive rates in wild boars and raccoon dogs tested for ADV were found to be 3.55% (8/225) and 0% (0/96), respectively. The presence of virus neutralization antibody titer against ADV means that small number of wild boars was infected with ADV and AD may be circulated continuously in Korean wild boar populations, and that wild boars may act as a potential reservoir of ADV. Therefore, to achieve the declaration of AD free, effective preventive measures to block transmission of AD should be taken to the wild boars.

Keywords: Sero-prevalence, Aujeszky's disease virus, Wild boar, Raccoon dogs

REFERENCES

1). Mettenleiter TC. Aujeszky's disease (pseudorabies) virus: the virus and molecular pathogenesis-state of the art, June 1999. Vet Res. 2000; 31:99–115.
2). Ferrari M, Gualandi GL, Corradi A, Monaci C, Romanelli MG, Tosi G, et al. Experimental infection of pigs with a thymidine kinase negative strain of pseudorabies virus. Comp Immunol Microbiol Infect Dis. 1998; 21:291–303.
crossref
3). Müller T, Bätza HJ, Schlüter H, Conraths FJ, Mettenleiter TC. Eradication of Aujeszky's disease in Germany. J Vet Med B Infect Dis Vet Public Health. 2003; 50:207–13.
4). Song JY, Lee JB, Hyun BH, Park JH, Kim BH, Kweon CH, et al. Glycoprotein g III of Ajesky's disease virus expressed in insect cells by a baculovirus. J Korean Soc Virol. 1992; 22:119–28.
5). Müller T, Hahn EC, Tottewitz F, Kramer M, Klupp BG, Mettenleiter TC, et al. Pseudorabies virus in wild swine: a global perspective. Arch Virol. 2011; 156:1691–705.
crossref
6). Paes Rde C, Fonseca AA Jr, Monteiro LA, Jardim GC, Piovezan U, Herrera HM, et al. Serological and molecular investigation of the prevalence of Aujeszky's disease in feral swine (Sus scrofa) in the subregions of the Pantanal wetland, Brazil. Vet Microbiol. 2013; 165:448–54.
7). Boadella M, Gortázar C, Vicente J, Ruiz-Fons F. Wild boar: an increasing concern for Aujeszky's disease control in pigs? BMC Vet Res. 2012; 17(8):7.
crossref
8). Maresch C, Lange E, Teifke JP, Fuchs W, Klupp B, Müller T, et al. Oral immunization of wild boar and domestic pigs with attenuated live vaccine protects against Pseudorabies virus infection. Vet Microbiol. 2012; 161:20–5.
crossref
9). Sedlak K, Bartova E, Machova J. Antibodies to selected viral disease agents in wild boars from the Czech Republic. J Wildl Dis. 2008; 44:777–80.
crossref
10). Montagnaro S, Sasso S, De Martino L, Longo M, Iovane V, Ghiurmino G, et al. Prevalence of antibodies to selected viral and bacterial pathogens in wild boar (Sus scrofa) in Campania Region, Italy. J Wildl Dis. 2010; 46:316–9.
11). Cramer SD, Campbell GA, Njaa BL, Morgan SE, Smith SK 2nd, McLin WR 4th, et al. Pseudorabies virus infection in Oklahoma hunting dogs. J Vet Diagn Invest. 2011; 23:915–23.
crossref
12). OIE (Office International des epizooties). 2012 Aujeszky's disease. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (Mammals, Birds and Bees). 7th ed.Paris: OIE;;2012. p. 97–111.
13). Vicente J, León-Vizcaíno L, Gortázar C, José Cubero M, González M, Martín-Atance P. Antibodies to selected viral and bacterial pathogens in European wild boars from southcentral Spain. J Wildl Dis. 2002; 38:649–52.
crossref
14). Martino PE, Montenegro JL, Preziosi JA, Venturini C, Bacigalupe D, Stanchi NO, et al. Serological survey of selected pathogens of free-ranging foxes in southern Argentina, 1998–2001. Rev Sci Tech. 2004; 23:801–6.
crossref
15). Ruiz-Fons F, Vidal D, Höfle U, Vicente J, Gortázar C. Aujeszky's disease virus infection patterns in European wild boar. Vet Microbiol. 2007; 120:241–50.
crossref
16). Platt KB, Graham DL, Faaborg RA. Pseudorabies: experimental studies in raccoons with different virus strains. J Wildl Dis. 1983; 19:297–301.
crossref
17). Weigel RM, Hahn EC, Scherba G. Survival and immunization of raccoons after exposure to pseudorabies (Aujeszky's disease) virus gene-deleted vaccines. Vet Microbiol. 2003; 92:19–24.
crossref
18). Elbers AR, Tielen MJ, Cromwijk WA, Hunneman WA. Sero-epidemiological screening of pig sera collected at the slaughterhouse to detect herds infected with Aujeszky's disease virus, porcine influenza virus and Actinobacillus (Haemophilus) pleuropneumoniae in the framework of an integrated quality control (IQC) system. Vet Q. 1990; 12:221–30.
19). Pensaert M, Labarque G, Favoreel H, Nauwynck H. Aujeszky's disease vaccination and differentiation of vaccinated from infected pigs. Dev Biol (Basel). 2004; 119:243–54.

Figure 1.
The number of Aujeszky's disease cases in Korea between 1995 and 2013.
jbv-44-336f1.tif
Table 1.
Seroprevalence rate of ADV by neutralization assay in blood samples of wild boars and raccoon dogs
Animals Positive Negative Positive rate (%)
Wild boars 8 217 3.55 (8/225)
Raccoon dogs 0 96 0 (0/96)
Table 2.
Regional distribution of Aujeszky's disease virus antibody from Korean wild boars and raccoon dogs
Animals Province
 Total
GW* GG GS CC JL
Wild boars (%) 0/37 (0) 0/45 (0) 1/61 (1.64) 6/70 (8.57) 1/12 (8.33) 8/225 (3.55)
Raccoon dogs (%) 0/74 (0) 0/22 (0) − − − 0/96 (0)

* GW: Ganwon-do, GG: Gyeonggi-do, GS: Gyeongsang-do, CC: Chungcheong-do, and JR: Jeolla-do.

Table 3.
Distribution of virus neutralizing antibody titers against ADV in Korean wild boars and raccoon dogs
Animals No. of samples Virus neutralizing antibody titer against ADV
<1:2 1:2 1:4 1:8 1:16 1:32 1:64 1:128
Wild boars 225 217 0 2 1 2 0 1 2
Raccoon dogs 96 96 0 0 0 0 0 0 0
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