Journal List > J Bacteriol Virol > v.44(3) > 1034141

Kim, Lee, Lee, Park, Ko, and Kim: Human Adenovirus Type 5 as a Delivery Vector is Not Neutralized in Field Serum Samples of Cattle, Pig, and Goat of Republic of Korea

Abstract

Human adenovirus type 5 (hAd5) vectors have been demonstrated to be useful vehicles for gene expressions in animals. However, it has not been reported whether hAd5 transduction might be hampered in the sera of livestock animals in Republic of Korea. We collected 205 samples of livestock animals, such as pig (n=84), cattle (n=84), and goat (n=37) in Korea. The neutralizing antibody (NAb) titers to hAd5 virus were less than 15 in most of samples. Only 8% of goat samples had a NAb titer of 15 or 30. Thus, we showed that hAd5 virus was not neutralized in sera from cattle, pig, and goat, and suggest that the hAd5 vector could be used for the effective delivery of vaccines or proteins in livestock animals in the field.

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Figure 1.
Distribution of serum samples from cattle, pig and goat according to neutralizing antibody to human adenovirus type 5 virus (hAd5). The virus neutralizing antibody titer was estimated from the reciprocal of the highest serum dilution that completely prevented the development of a cytopathic effect (CPE). The percent of serum samples that display hAd5-specific neutralization titers of <15, 15∼50, 50∼100, and >100 are shown. The rabbit polyclonal antibody to adenovirus type 5 virus (Abcam ab6982, USA) was used as a positive serum control.
jbv-44-269f1.tif
Table 1.
Serum samples of cattle, pig, and goat used in this study
Province The number of samples for species
Cattle Pig Goat
Chungbuk 8 8 12
Gyeongnam 12 12 8
Jeju 8 8
Gyeonggi 12 12
Gyeongbuk 28 16 9
Jeonbuk 16 16 8
Gangwon 12
Total 84 84 37
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