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Jin, Jung, Jeon, Choi, Kim, Oh, Choi, and Jung: Molecular Epidemiology of Clostridium perfringens Isolated from Food Poisoning in Seoul, 2013
Original Article

J Bacteriol Virol 2014;44(2):170-176.

Published online: 9 February 2014

DOI: https://doi.org/10.4167/jbv.2014.44.2.170

Molecular Epidemiology of Clostridium perfringens Isolated from Food Poisoning in Seoul, 2013

Younghee Jin*, Jihun Jung, Sujin Jeon, Seongseon Choi, Youngeun Kim, Younghee Oh, Sungmin Choi, Kweon Jung

Division of Infectious Disease, Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, Korea

*Corresponding author: Younghee Jin. Division of Infectious Disease, Seoul Metropolitan Government Research Institute of Public Health and Environment 202-3, Yangjae2-dong, Seocho-gu, Seoul 137-134, Korea. Phone: +82-2-570-3461, Fax: +82-2-570-3418, e-mail: jinyh94@seoul.go.kr

Received 31 March 20147 May 2014       Accepted 10 May 2014

Copyright © 2014 Journal of Bacteriology and Virology

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Clostridium perfringens food poisoning ranks among the most common gastrointestinal diseases in developed countries. In Korea, C. perfringens food poisoning gradually increases. Using PCR, 72 strains of C. perfringens isolated in Seoul, 2013 were tested for the presence of toxin genes. Of the tested strains, 32 isolates carried the cpe gene, 37 isolates carried the cpb2 gene and 3 isolates carried the cpe and cpb2 genes, respectively. 32 cpe- positive strains were isolated from the food poisoning patient, whereas among 37 cpb2-positive strains, 22 strains were isolated from asymptomatic person. To investigate epidemiological relationship between the isolates, Pulsed-filed gel electrophoresis (PFGE) was performed. The genetic relatedness of the isolates ranged from 55.9% to 100% and 47 distinct PFGE profiles were observed. The results show that the cpe- positive outbreak strains showed close genetic relation, whereas the cpb2-positive isolates revealed a wide genetic diversity.

Keywords: Clostridium perfringens, Toxin gene, PFGE

REFERENCES

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Figure 1.
Dendrogram showing the clustering of PFGE patterns for the 72 C. perfringens isolates. A group indicates 72.6% similarity; B group indicates 73.4% similarity; C group indicates 76.7% similarity; D group indicates 72.7% similarity. ST means PFGE subtype pattern.
jbv-44-170f1.tif
Table 1.
Isolation and region distribution of 72 C. perfringens isolated in Seoul, 2013
Case no. Receipt date Region (Gu) Occurrence place Isolation origin Toxin gene Isolation number
1 2013-3-8 Yeongdeungpo School Patient cpb2 1
2 2013-3-26 Gangdong School Patient cpe, cpb2
cpb2		 1
1
3 2013-4-24 Gangnam Restaurant Patient cpe 5
4 2013-4-26 Yongsan Restaurant Patient Foodservice employees cpe
cpe		 16
1
5 2013-5-2 Seodaemun Restaurant Patient cpb2 1
6 2013-5-13 Seodaemun Restaurant Foodservice employees
Patient		 cpb2
cpb2		 2
1
7 2013-5-20 Seodaemun Restaurant Patient cpb2 1
8 2013-5-28 Kwanak Restaurant Patient cpb2 1
9 2013-7-4 Kwanak Restaurant Patient
Foodservice employees		 cpb2
cpb2		 1
1
10 2013-7-4 Gwangjin Restaurant Foodservice employees cpb2 1
11 2013-7-12 Guro Restaurant Foodservice employees cpb2 1
12 2013-8-30 Geumcheon Restaurant Patient cpe, cpb2 1
13 2013-9-5 Jongno Restaurant Patient cpe 10
14 2013-10-1 Gangbuk Restaurant Patient cpb2 1
15 2013-10-30 Seongdong Restaurant Foodservice employees cpb2 2
16 2013-11-29 Mapo School Patient
Foodservice employees		 cpb2
cpb2		 3
1
17 2013-12-10 Seodaemun School Foodservice employees cpe, cpb2 1
18 2013-12-11 Dongdaemun Overseas trip Patient contact cpb2 1
19 2013-12-20 Dongjak School Patient cpb2 2
20 2013-12-20 Jongno School Patient cpb2 3
21 2013-12-23 Gangnam Restaurant Foodservice employees cpb2 2
23 2013-12-27 Gangnam Restaurant Patient cpb2 6
24 2013-12-27 Mapo Restaurant Foodservice employees Foodservice employees cpb2
cpb2		 1
3
Total 72
Table 2.
Distribution of toxin genes of 72 C. perfringens isolated in Seoul, 2013
Toxin genes Isolates from patients (%) Isolates from asymptomatic subjects (%) Total
cpe 32 (100) 0 (0) 32
cpb2 22 (59.5) 15 (40.5) 37
cpe & cpb2 2 (66.7) 1 (33.3) 3
Total 56 (77.8) 16 (22.2) 72
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