Possible Roles of UL112-113 Proteins in Human Cytomegalovirus DNA Replication

Young-Eui Kim; Jin-Hyun Ahn

doi:10.4167/jbv.2012.42.2.162

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Kim and Ahn: Possible Roles of UL112-113 Proteins in Human Cytomegalovirus DNA Replication

Letter to the Editor

Journal of Bacteriology and Virology

Journal of Bacteriology and Virology 2012; 42(2): 162-168.

Published online: 22 June 2012

DOI: https://doi.org/10.4167/jbv.2012.42.2.162

Possible Roles of UL112-113 Proteins in Human Cytomegalovirus DNA Replication

Young-Eui Kim, Jin-Hyun Ahn

Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Korea.

Corresponding author: Jin-Hyun Ahn. Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, 300 Cheoncheondong, Jangangu, Suwon, Gyeonggido 440-746, Korea. Phone: +82-31-299-6222, Fax: +82-31-299-6435, jahn@skku.edu

Received 7 April 2012 Revised 11 April 2012 Accepted 12 April 2012

Abstract

DNA replication of human cytomegalovirus (HCMV) is a highly regulated process that requires specific interactions between cis-acting lytic origin of replication (oriLyt) and trans-acting viral proteins. Formation of the replication initiation complex is also regulated by specific interactions among viral replication proteins. HCMV replication proteins include origin-binding proteins, core proteins that work in replication forks, and regulatory proteins that modulate host cell functions. This letter describes intriguing questions regarding how HCMV origin-binding proteins interact with oriLyt to initiate DNA replication and how the regulatory UL112-113 proteins, which are found only in beta-herpesviruses, function to promote viral DNA replication.

Keywords: HCMV, DNA replication, UL112-113

Figures and Tables

Figure 1

HCMV-encoded replication proteins, the structure of oriLyt, and a possible role of UL112-113 proteins in the formation of the replication initiation complex. (A) The HCMV genome consisting of unique long (U_L) and unique short (U_S) sequences bound by terminal repeats (a/a', b/b', and c/c') and the position of open reading frames (ORFs) for replication proteins are shown. (B) Schematic of HCMV oriLyt showing the relative position of two essential regions, oriLyt promoter, small replicator transcript (SRT), binding sites for C/EBPα and IE2, a highly pyrimidine-rich sequence (Y-block) and the RNA stem-loop structure. Nucleotide (nt) numbers shown are with reference to the AD169 strain DNA sequence. (C) A possible role of UL112-113 proteins as a mediator between the origin-binding complex and the polymerase complex in the formation of the replication initiation complex.

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