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Lee, Kim, Cho, Song, Song, Lee, Kang, Baik, Cho, Rhee, Seo, Youn, and Lee: Comparison of Proteome Components of Helicobacter pylori Before and After Mouse Passage
Original Article

J Bacteriol Virol 2011;41(4):267-278.

Published online: 9 February 2011

DOI: https://doi.org/10.4167/jbv.2011.41.4.267

Comparison of Proteome Components of Helicobacter pylori Before and After Mouse Passage

Kyoung-Ja Lee1, Bok-Ran Kim1, Young-A Cho1, Yun-Gyu Song1, Jae-Young Song1, 3, Kon Ho Lee1, 3, Hyung-Lyun Kang1, 4, Seung-Chul Baik1, 3, 4, Myung-Je Cho1, 3, 4, Kwang-Ho Rhee1, 4, Ji-Hyun Seo2, Hee-Shang Youn2, 3, Woo-Kon Lee1, 3, 4, *

1Department of Microbiology, Gyeongsang National Univerity School of Medicine, Jinju, Korea

2Department of Pediatrics, Gyeongsang National Univerity School of Medicine, Jinju, Korea

3Department of Biomedical Center (BK21), Gyeongsang National Univerity School of Medicine, Jinju, Korea

4Institute of Health Sciences, Gyeongsang National Univerity, Jinju, Korea

*Corresponding author: Woo-Kon Lee, VMD, PhD. Department of Microbiology, Gyeongsang National University School of Medicine, Jinju, Gyeong-Nam 660-751, Korea. Phone: +82-55-772-8083, Fax: +82-55-772-8089 e-mail: wklee@gnu.ac.kr

** This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (521-2007-1-E00035).

Received 27 October 201119 November 2011       Accepted 29 November 2011

Copyright © 2011 Journal of Bacteriology and Virology

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The mouse model is alleged to be a useful tool for understanding of pathophysiological roles of Helicobacter pylori in the development of gastric disorders. However, it has been observed that H. pylori strains significantly differed in their fitness in mice and even mouse strains differed in their susceptibilities to a H. pylori strain. Bacterial components of H. pylori which could affect on its fitness in mice have to be elucidated for the establishment of the mouse model for H. pylori infections. In the comparison of colonization ability between two H. pylori Korean isolates, 51 (isolated from a patient with duodenal ulcer) and 52 (isolated from a patient with gastric cancer), 52 could colonize better than 51 on the gastric mucosa of mouse. Proteome components of H. pylori 52, as a good colonizer and H. pylori 51, as a poor one were quantitatively compared each other. Five bacterial proteins including catalase, urease subunit alpha/beta, enolase and ferritin, were up-regulated in 52. In addition, the respective proteome components of the two strains were also compared with their mouse-passaged homologous strains. Seven and five proteins, which included catalase, flagellin A/B in common, were up-regulated in mouse-adapted 51 and 52, respectively. Among the fourteen identified proteins, urease subunit alpha/beta, flagellin A/B, catalase, ferritin, superoxide dismutase and neutrophil-activation protein have been previously known to be necessary to gastric colonization of H. pylori in animal models. The other up-regulated proteins including enolase, elongation factor Tu and fructose-bisphosphate aldolase have been reported to be associated with acid tolerance of H. pylori. These data provide confirmatory evidence for the importance of those proteins in the development of H. pylori-associated gastric disorders.

Keywords: Helicobacter pylori, Proteome, Colonization, Mouse passage

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Figure 1.
Mouse colonization abilities of H. pylori 51 and 52. Mice were inoculated with H. pylori twice for two consecutive days with a dose of 2 × 109 cells. Mice were sacrificed 2 weeks later, antrum and corpus were separated and H. pylori was examined from each tissue by plate counts. Each spot represents the CFU count from one mouse. According to Mann-Whitney U test analysis, there is a significant difference between 51 and 52 in FVB corpus colonization ability (p = 0.04). The horizontal bars within each group represent the median value.
jbv-41-267f1.tif
Figure 2.
Two-dimensional gel electrophoresis profiles of H. pylori 51 and 52. A total 100 μg of whole-cell protein of each strain was applied on 170-mm IPG strips with a range of pH 3–10, followed by 12% SDS-PAGE, and visualized by silver staining. The bold numbers with thick arrows represent proteins that are strong in spot intensity compared to the other strain.
jbv-41-267f2.tif
Figure 3.
Comparison of two-dimensional gel electrophoresis profiles of H. pylori 52 before and after FVB mouse passage. 100 μg of whole-cell protein of each strain was applied on 170-mm IPG strips with a range of pH 3∼10, followed by 12% SDS-PAGE, and visualized by silver staining. The bold numbers with thick arrows represent proteins that are up-regulated after mouse passage.
jbv-41-267f3.tif
Figure 4.
Comparison of two-dimensional gel electrophoresis profiles of H. pylori 51 before and after FVB mouse passage. 100 μg of whole-cell protein of each strain was applied on 170-mm IPG strips with a range of pH 3∼10, followed by 12% SDS-PAGE, and visualized by silver staining. The bold numbers with thick arrows represent proteins that are up-regulated after mouse passage.
jbv-41-267f4.tif
Table 1.
Identification of differentially expressed proteins in the whole-cell protein fractions of H. pylori 51 and 52
Spot No.* Protein description Gene codes MW (Da) / pI Spot intensity (n=3, mean ± SD)
 Strain with up-regulation (fold variation)
51 52
1 Urease subunit beta HP0072 61,683/5.9 498.40±376.97 1367.38±1013.2 52 (2.21)
2 Catalase HP0875 58,630/8.7 57.14±59.41 185.74±51.35 52 (3.31)
4 Enolase HP0154 46,562/5.5 18.63±32.26 180.33±18.31 52 (9.68)
5 Urease subunit alpha HP0073 26,539/8.9 203.61±352.66 509.02±194.70 52 (2.50)
8 Ferritin HP0653 1,9286/5.6 150.12±134.92 667.72±298.33 52 (4.45)
3 Glutamine synthetase HP0512 54,513/6.0 67.55±28.73 3.91±6.78 51 (17.28)
6 Superoxide dismutase HP0389 24,518/6.0 863.13±53.83 417.63±64.16 51 (2.07)
7 ATP-dependent protease binding subunit HP0264 96,638/6.2 298.51±163.37 43.08±74.61 51 (6.93)
9 Neutrophil activating protein HP0243 16,933/5.8 1404.04±309.76 324.69±283.82 51 (4.09)

* Spot numbers are shown in Fig. 2.

Table 2.
Identification of up-regulated proteins in the whole-cell protein fractions of H. pylori 52 after FVB mouse passage
Spot No. Protein description Gene codes MW (Da) / pI Spot intensity (n=3, mean ± SD)
 Fold variation
before passage after passage
1 Flagellin B HP0115 53,882/6.1 9.05±15.68 21.82±18.90 2.41
2 Flagellin A HP0601 53,284/6.3 54.44±49.15 175.89±152.20 3.23
3 Catalase HP0875 58,630/8.7 185.74±51.35 404.71±282.79 2.18
4 3-dehydroquinate dehydratase HP1038 18,483/4.9 35.25±61.05 154.76±134.09 4.39
5 Neutrophil activating protein HP0243 16,933/5.8 324.69±283.82 965.66±836.30 2.98

* Spot numbers are shown in Fig. 3.

Table 3.
Identification of up-regulated proteins in the whole-cell protein fractions of H. pylori 51 after FVB mouse passage
Spot No. Protein description Gene codes MW (Da) / pI Spot intensity (n=3, mean ± SD)
 Fold variation
Before passage After passage
1 Urease subunit beta HP0072 61,683/5.9 498.40±376.97 1029.54±827.08 2.06
2 Flagellin B HP0115 53,882/6.1 16.98±25.50 61.41±13.54 3.62
3 Flagellin A HP0601 53,284/6.3 168.68±111.58 336.54±159.49 2.00
4 Catalase HP0875 58,630/8.7 57.14±59.41 212.16±139.34 3.71
5 Elongation factor Tu HP1205 43,648/5.2 46.50±11.78 151.06±22.60 3.25
6 Fructose-bisphosphate aldolase HP0176 33,772/6.2 12.28±21.27 117.30±47.24 9.55
7 Urease subunit alpha HP0073 26,539/8.9 203.61±352.66 880.33±535.57 4.33

* Spot numbers are shown in Fig. 4.

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