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Abstract
Avian influenza (AI) is an infectious, low pathogenic virus that is endemic all over the world and poses a potential threat to the poultry industry. Vaccination is a widely used effective method to prevent avian influenza virus. Here we employed a comparative proteomics approach [two-dimensional electrophoresis (2-DE) and matrix assisted laser desorption ionization-time of flight (MALDI-TOF)] to characterize proteome in the sera from the specific pathogen free (SPF) chickens, the vaccinated chickens, and the naturally infected chickens. We identified total 58 proteins that were differentially expressed in the sera of three groups. Among them ovotransferrin and vitamin D-binding protein were more expressed in the sera of naturally infected chickens compare with other groups. Our results suggested that the level of these two proteins in the serum may help to discriminate the naturally infected chicken from the vaccinated chicken.
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 | Figure 1.A representative result of the analysis of the protein expression in the SPF chicken serum (18 week) and the vaccinated chicken serum (18 week). 100 μg of proteins from the SPF chicken serum (A) and the vaccinated chicken serum (B) were separated by IEF using linear pH gradient strip of pH 4~7 and 12% SDS-PAGE gels. The numbers represent unique numbers automatically assigned to each protein spot by PDQuest2D analysis software. |
 | Figure 2.A representative result of the analysis of the protein expression in the SPF chicken serum (7~8 week) and the infected chicken serum (7~8 week). 100 μg of proteins from the SPF chicken serum (A) and the infected chicken serum (B) were separated by IEF using linear pH gradient strip of pH 4~7 and 12% SDS-PAGE gels. The numbers represent unique numbers automatically assigned to each protein spot by PDQuest 2D analysis software. |
 | Figure 3.Overexpressed proteins in the infected chicken serum compare with the SPF chicken serum. (A) Ovotransferrin. (B) Vitamin D-binding protein (DBP). |
Table 1.
Differentially expressed proteins list between the SPF chicken serum and the vaccinated chicken serum
Est'd Z score is a chance value used in ProFound that corresponds to the percentile of the search in a random match population. For instance, a Z score of 1.65 for a search indicates that the search is in the 95th percentile. In the intensity, a dash (–) means that the protein is not expressed in the group.
Table 2.
Differentially expressed proteins list between the SPF chicken serum and the infected chicken serum
Est'd Z score is a chance value used in ProFound that corresponds to the percentile of the search in a random match population. For instance, a Z score of 1.65 for a search indicates that the search is in the 95th percentile. In the intensity, a dash (–) means that the protein is not expressed in the group.
Table 3.
Differentially expressed proteins list between the vaccinated chicken serum and the infected chicken serum
Est'd Z score is a chance value used in ProFound that corresponds to the percentile of the search in a random match population. For instance, a Z score of 1.65 for a search indicates that the search is in the 95th percentile. In the intensity, a dash (–) means that the protein is not expressed in the group.
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