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Lee and Kim: Epidemiological Analysis of Shiga Toxin-producing E. coli Isolated in Gwangju, Korea, by Pulse-field Gel Electrophoresis
Original Article

J Bacteriol Virol 2009;39(3):195-203.

Published online: 18 January 2009

DOI: https://doi.org/10.4167/jbv.2009.39.3.195

Epidemiological Analysis of Shiga Toxin-producing E. coli Isolated in Gwangju, Korea, by Pulse-field Gel Electrophoresis

Jeong-Chi Lee1, Min Ji Kim2, ∗

1Department of Clinical Pathology, Gwangju Health College University, Gwangju, Korea

2Health & Environment Institute of Gwangju, Gwangju, Korea

∗Corresponding author: Min Ji Kim. Health & Environment Institute of Gwangju, 898 Hwajung-dong, Seo-gu, Gwangju 502-837, Korea. Phone: +82-62-380-1837, Fax: +82-62-380-1836 e-mail: vetmj@korea.kr

∗∗ This research was performed as a part of the laboratory surveillance of acute diarrhea, managed by the National Institute of Health, Republic of Korea.

      Revised 14 May 20098 June 2009       Accepted 11 June 2009

Copyright © 2009 Korean Society for Legal Medicine

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In Gwangju, Korea, over the last 4 years, human gastrointestinal infection caused by shiga toxin-producing E. coli (STEC) increased. The aim of this study was to ascertain the genetic relatedness of STEC strains by pulsed-field gel electrophoresis (PFGE), as no data on the molecular epidemiology of STEC in Gwangju has yet been published. The PFGE banding patterns were defined for 62 of the 67 STEC strains isolated from cattle and human. There were 11 clonal types in the 11 STEC strains of cattle origin. Among the 11 STEC strains from asymptomatic person, four O91 strains were 100% similarity in band profiles. In the STEC strains isolated from diarrhea patients, same serogroups were grouped to the same cluster; O111 stains were 89.5% similarity, O157 strains 80%, O26 strains 81.5%, and O103 strains 91% similarity, respectively. In conclusion, this is the first report that a large collection of STEC strains from Korea has been analyzed, and a high degree of diversity was observed among the strains analyzed by this technique. PFGE analysis revealed that the strains isolated from human and cattle were closely related within serotypes, and it was useful for epidemiological analysis of STEC. The importance and usefulness of active laboratory surveillance of STEC such as PFGE should be recommended.

Keywords: Shiga toxin-producing E. coli, Pulsed-field gel electrophoresis, Cattle, Human

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Figure 1.
Dendrogram of XbaI-digested PFGE profiles of the STEC isolates from cattle.
jbv-39-195f1.tif
Figure 2.
Dendrogram of XbaI-digested PFGE profiles of the STEC isolates from asymptomatic patients.
jbv-39-195f2.tif
Figure 3.
Dendrogram of XbaI-digested PFGE profiles of the STEC isolates from diarrheagenic patients.
jbv-39-195f3.tif
Figure 4.
Dendrogram of XbaI PFGE banding pattern of each of the 67 STEC strains clustered by MPGMA using the Dice coefficient in Bionumerics version 5.0.
jbv-39-195f4.tif
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