Epidemiological Surveillance of P and G Genotypes of Group A Rotavirus Detected from Diarrheic Patients in Daejeon Region

Sun-Kyoung Park; Young-Sun Heo; Sun-Hye Bing; Doo-Sung Cheon; Jiaqi Chu; Moo-Hyung Jun

doi:10.4167/jbv.2007.37.3.169

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Park, Heo, Bing, Cheon, Chu, and Jun: Epidemiological Surveillance of P and G Genotypes of Group A Rotavirus Detected from Diarrheic Patients in Daejeon Region

Original Article

J Bacteriol Virol 2007;37(3):169-175.

Published online: 18 February 2007

DOI: https://doi.org/10.4167/jbv.2007.37.3.169

Epidemiological Surveillance of P and G Genotypes of Group A Rotavirus Detected from Diarrheic Patients in Daejeon Region

Sun-Kyoung Park, Young-Sun Heo, Sun-Hye Bing, Doo-Sung Cheon¹, Jiaqi Chu², Moo-Hyung Jun²

Daejeon Metropolitan City Institute of Health & Environment, Daejeon, Korea

¹Korea Center for Disease Control, Seoul, Korea

²College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea

Received 24 November 2006 Accepted 25 July 2007

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

During 3 years surveillance (January 2001 through December 2003) for acute gastroenteritis in human in Daejeon region, 432 out of 4,869 stool samples were selected as rotavirus-positive specimens by means of antigen-capture enzyme-linked immunosorbent assay (ELISA). The P (VP4) and G (VP7) genotypes for 432 stool samples were investigated by reverse transcription polymerase chain reaction (RT-PCR) and nested multiplex PCR. The most prevalent P subtype was P[8] (44.9%), followed by P[4] (25.7%) and P[6] (17.1%). No cases for P[10] and P[9] subtypes were found through the study. In G subtyping, G1 (53.2%) was the most frequently found G type, followed by G2 (23.1%), G3 (9.5%), G4 (6.7%), and G9 (0.9%). The order of detection rates for G2, G3 and G4 was variable by years. The most common G- and P- type combination found in this study was G1P[8] (33.1%), followed by G2P[4] (20.4%), G1P[6] (10.0%), G3P[8] (7.2%) and G4P[6] (4.2%). The mixed types of G and P were observed most frequently in P[8] (1.4%) and G1 (3.2%), respectively. This is the first molecular epidemiological study for Group A rotavirus in Daejeon region. The results might be useful data for evaluating the epidemiological status of rotaviral diarrhea in the region.

Keywords: Rotavirus, Acute gastroenteritis, P and G genotypes, Nested multiplex PCR

References

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Figure 1.

Patterns of nested multiplex PCR for P and G genotypes of Group A rotavirus detected from stool specimens.

(A) Lane M; 100 bp DNA ladder, Lanes 1; P[8], 2; P[4], 3: P[6].

(B) Lane M; 100 bp DNA ladder, Lanes 1; G1, 2; G2, 3; G3, 4; G9, 5; G4.

Table 1.

Distribution of P and G genotypes of rotavirus detected from stool samples of diarrheic patients in Daejeon region during 2001∼2003

	Types	G							Total
	Types	G1	G2	G3	G4	G9	Mixed	Nontypeable	Total
P	P[8]	143 (33.1)	3 (0.7)	21 (7.2)	10 (2.3)	2 (0.5)	6 (1.4)	9 (2.1)	194 (44.9)
	P[4]	12 (2.8)	88 (20.4)	6 (1.4)	1 (0.2)	0 (0.0)	3 (0.7)	1 (0.2)	111 (25.7)
	P[6]	43 (10.0)	3 (0.7)	5 (1.2)	18 (4.2)	2 (0.5)	1 (0.2)	2 (0.5)	74 (17.1)
	P[10]	0 (0.0)	0 (0.0)	0 (0.0)	0 (0.0)	0 (0.0)	0 (0.0)	0 (0.0)	0 (0.0)
	P[9]	0 (0.0)	0 (0.0)	0 (0.0)	0 (0.0)	0 (0.0)	0 (0.0)	0 (0.0)	0 (0.0)
	Mixed	14 (3.2)	2 (0.5)	1 (0.2)	0 (0.0)	0 (0.0)	1 (0.2)	0 (0.0)	18 (4.2)
	Nontypeable	14 (3.2)	4 (0.9)	8 (1.9)	0 (0.0)	0 (0.0)	1 (0.2)	8 (1.9)	35 (8.1)
	Total	226 (52.3)	100 (23.1)	41 (9.5)	29 (6.7)	4 (0.9)	12 (2.8)	20 (4.6)	432 (100.0)

( ) = %; No. of types/Total No. of specimens × 100

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