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Ahn, Cho, Paik, Lee, and Choi: Production of TNF-α and IL-6 in Macrophages by Mycobacterial Protein Antigens
Original Article

Immune Network 2007; 7(1): 26-30.

Published online: 31 March 2007

DOI: https://doi.org/10.4110/in.2007.7.1.26

Production of TNF-α and IL-6 in Macrophages by Mycobacterial Protein Antigens

Hae-Jeong Ahn1, Sang-Nae Cho1, Tae-Hyun Paik2, Junglim Lee2, In-Hong Choi1

1Department of Microbiology, Yonsei University College of Medicine, Seoul, Korea.

2Department of Microbiology, College of Medicine, Konyang University, Daejeon, Korea.

Corresponding author (inhong@yumc.yonsei.ac.kr)

Copyright © 2007 The Korean Association of Immunologists

(open-access):

This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Mycobacterial antigens released as PIM, LM, LAM, lipoproteins and other cellular factors may contribute to macrophage and dendritic cell activation through pattern recognition receptors such as TLRs. In this study, we assessed cytokine production and ERK activation with stimulation of several major mycobacterial antigens.

Methods

Purified mycobacterial antigens (10, 22, 30, 38kDa) and recombinant antigens (6, 16, 19, 38kDa, Ag85A antigen) were studied. The production of cytokines (TNF-α, IL-12, IL-6) was measured by ELISA. The ERK activation was detected by western blotting. The expression of TLR2 or TLR4 was measured by flow cytometry.

Results

Among purified antigens only 30kDa antigen induced production of IL-6 or TNF-α in THP-1 macrophage cells. When THP-1 macrophage cells were treated with 30kDa antigen, phosphorylation of ERK was detected. ERK activation also occurred in TLR2 transfectant HEK293 cells with 30kDa antigen stimulation.

Conclusion

30kDa antigen is one of the major mycobacterial antigens inducing cytokine production and MAP kinases phosphorylation in macrophages.

Keywords: Macrophage, M. tuberculosis, 30kDa antigen, TNF-α, IL-6, ERK

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