Journal List > Immune Netw > v.7(3) > 1033754

TOOLS
Kim, Ko, Kim, Han, Lee, Chang, Jin, Sung, and Kang: B Cells Transduced with HPV16 E6/E7-expressing Adenoviral Vector Can Efficiently Induce CTL-dependent Anti-Tumor Immunity
Original Article

Immune Network 2007; 7(3): 109-116.

Published online: 30 September 2007

DOI: https://doi.org/10.4110/in.2007.7.3.109

B Cells Transduced with HPV16 E6/E7-expressing Adenoviral Vector Can Efficiently Induce CTL-dependent Anti-Tumor Immunity

Yun-Sun Kim1, Hyun-Jeong Ko1, Yeon-Jeong Kim1, Seung-Hee Han1, Jung-Mi Lee1, Woo-Sung Chang1, Hyun-Tak Jin2, Young-Chul Sung2, Chang-Yuil Kang1

1Laboratory of Immunology, Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul, Korea.

2Division of Molecular and Life Sciences, Postech Biotech Center, Pohang University of Science & Technology, Pohang, Korea.

Correspondence to: Chang-Yuil Kang, Laboratory of Immunology, Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, 59, Gwanakno, Gwanak-gu, Seoul 151-742, Korea. (Tel) 82-2-880-7860, (Fax) 82-2-885-1373, cykang@snu.ac.krcykang@snu.ac.kr

Copyright © 2007 The Korean Association of Immunologists

(open-access):

This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Human papillomavirus (HPV) infection is responsible for cervical cancer, a common cancer in women. Since HPV infection and cancer development are controlled by the host immune system, immunotherapy against HPV can be helpful in preventing or treating HPV-associated cervical cancer. Two oncoproteins of HPV16, E6 and E7, are promising targets for immunotherapy against cervical cancer, because they are constitutively expressed in cervical cancer.

Methods

Since cellular vaccines using B cells as well as dendritic cells offer an efficient approach to cancer immunotherapy, we opted to use B cells. We evaluated the immunogenicity and anti-tumor effects of a B cell vaccine transduced with HPV16 E6/E7-expressing adenovirus.

Results

Vaccination with HPV16 E6/E7-transduced B cells induced E6/E7-specific CD8+ T cell-dependent immune responses and generated anti-tumor effects against E6/E7-expressing TC-1 tumor. The anti-tumor effect induced by this B cell vaccine was similar to that elicited by DC vaccine, showing that B cells can be used as an alternative to dendritic cells for cellular vaccines.

Conclusion

Thisstudy has shown the feasibility of using B cells as immunogenic APCs and the potential for developing prophylactic and therapeutic vaccines against HPV-associated cervical cancer using a B cell vaccine transduced with adenovirus expressing HPV16 E6/E7.

Keywords: HPV16 E6/E7, adenoviral vector, B cells, anti-tumor immunity

TOOLS
Similar articles