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Cho, Chwae, Park, and Kim: Association of Killer Cell Ig-like Receptor (KIR) with an Adaptor Protein Shc
Original Article

Immune Network 2006; 6(2): 67-75.

Published online: 30 June 2006

DOI: https://doi.org/10.4110/in.2006.6.2.67

Association of Killer Cell Ig-like Receptor (KIR) with an Adaptor Protein Shc

Hyun Il Cho1, Yong-Joon Chwae1, Sang Myun Park1, Jongsun Kim1, 2

1Department of Microbiology, Yonsei University College of Medicine, Seoul, Korea.

2Brain Korea Project of Medical Sciences,Yonsei University College of Medicine, Seoul, Korea.

Correspondence to: Jongsun Kim, Department of Microbiology and Institute for Immunology and Immunological Diseases, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-752, Korea. (Tel) 82-2-2228-1814, (Fax) 82-2-392-7088, jkim63@yumc.yonsei.ac.kr

Copyright © 2006 The Korean Association of Immunologists

(open-access):

This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Cytotoxic function of killer cells is inhibited by specific recognition of class I MHC molecules on target cells by inhibitory killer Ig-like receptors (KIR) expressed on NK cells and some cytotoxic T cells. The inhibitory effect of KIR is accomplished by recruitment of SH2-containing protein tyrosine phosphatase (SHP) to the phosphotyrosine residues in the cytoplasmic tail.

Methods

By in vitro coprecipitation experiments and transfection analysis, we investigated the association of KIR with an adaptor protein Shc in Jurkat T cells.

Results

The cytoplasmic tail of KIR appeared to associate with an adaptor protein Shc in Jurkat T cell lysates. Similar in vitro experiments showed that phosphorylated KIR cytoplasmic tail bound SHP-1 and Shc in Jurkat T cell lysates. The association of KIR with Shc was further confirmed by transfection analysis in 293T cells. Interestingly, however, Shc appeared to be replaced by SHP-2 upon engagement of KIR in 293T cells.

Conclusion

Our data indicate that KIR associate with an adaptor protein Shc in Jurkat T cells, and suggest that KIR might have an additional role which is mediated by this adaptor protein.

Keywords: KIR, Shc, SHP-1, SHP-2, cell activation, cell proliferation, inhibitory signal transduction

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