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Yeo, Cha, and Park: Differences in Their Proliferation and Differentiation between B-1 and B-2 Cell
Original Article

Immune Network 2006; 6(1): 1-5.

Published online: 31 March 2006

DOI: https://doi.org/10.4110/in.2006.6.1.1

Differences in Their Proliferation and Differentiation between B-1 and B-2 Cell

Seung Geun Yeo1, Chang Il Cha1, Dong Choon Park2

1Department of Otolaryngology, College of Medicine, Kyung Hee University, Seoul, Korea.

2Department of Obstetric and Gynecology, St. Vincent's Hospital, The Catholic University of Korea, Suwon, Korea.

Correspondence to: Dong Choon Park, Department of Obstetrics and Gynecology, St. Vincent's Hospital, The Catholic University of Korea, 93 Gi-dong, Paldal-gu, Suwon, Korea. (Tel) 82-31-244-9504, (Fax) 82-31-254-7481, dcpark@catholic.ac.kr

Copyright © 2006 The Korean Association of Immunologists

(open-access):

This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

B cell subset has been divided into B-1 cells and B-2 cells. B-1 cells are found most prominently in the peritoneal cavity, as well as constituting a small proportion of splenic B cells and they are larger and less dense than B-2 cells in morphology. This study was designed to compare the differences in their proliferation and differentiation between B-1 and B-2 cell.

Methods

We obtained sorted B-1 cells from peritoneal fluid and B-2 cells from spleens of mice. Secreted IgM was measured by enzyme-linked immunosorbent assay. Entering of S phase in response to LPS-stimuli was measured by proliferative assay. Cell cycle analysis by propidium iodide was performed. p21 expression was assessed by real time PCR.

Results

Cell proliferation and cell cycle progression in B-1 and B-2 cells, which did not occur in the absence of LPS, required LPS stimulation. After LPS stimulation, B-1 and B-2 cells were shifted to S and G2/M phases. p21 expression by resting B-1 cells was higher than that of resting B-2 cells.

Conclusion

B-1 cells differ from conventional B-2 cells in proliferation, differentiation and cell cycle.

Keywords: B-1 cell, B-2 cell, immunology, differentiation, cell cycle, p21

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