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Lee, Woo, Park, and Kwon: A Combination of PG490 and Lipopolysaccharide Induce Apoptosis through Activation of Casapase-3 and Down-regulation of cIAP1 and XIAP in Human Astroglioma Cell

Abstract

Background

Malignant gliomas are the most common primary tumors in the central nervous system.

Methods

We investigated the combined effect of PG490 and LPS on the induction of the apoptotic pathway in human astroglioma cells.

Results

Treatment of U87 cells with combination of 50 nM of PG490 and 50µg/ml of LPS resulted in increased internucleosomal DNA fragmentation, cleavage of PLC-γ1, and downregulation of cIAP1 and XIAP. The combination of LPS and PG490 treatment-induced apoptosis is mediated through the activation of caspase, which is inhibited by the caspase inhibitor, z-VAD-fmk. Also, release of cytochrome c was found in PG490 and LPS-cotreated U87 cell.

Conclusion

Taken together, combination of PG490 and LPS appears to be a potent inducer of apoptosis in astrogliaoma cells, and might have some benefit in the treatment of glioma patients.

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