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Cheon, Bang, and Cho: Inhibition of Cell Migration by Corticotropin-Releasing Hormone (CRH) in Human Natural Killer Cell Line, NK-92MI
Original Article

Immune Network 2005; 5(4): 247-251.

Published online: 31 December 2005

DOI: https://doi.org/10.4110/in.2005.5.4.247

Inhibition of Cell Migration by Corticotropin-Releasing Hormone (CRH) in Human Natural Killer Cell Line, NK-92MI

Soyoung Cheon1, 2, Saik Bang2, Daeho Cho1

1Department of Life Science, Sookmyung Women's University, Seoul, Korea.

2Department of Plastic Surgery, Samsung Medical Center, Sunhkyunkwan University School of Medicine, Seoul, Korea.

Corresponding author (cdhkor@sookmyung.ac.kr)

Copyright © 2005 The Korean Association of Immunologists

(open-access):

This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Natural killer (NK) cells are CD3 (-) CD14 (-) CD56 (+) lymphocytes. They play an important role in the body's innate immune response. They can induce spontaneous killing of cancer cells or virus-infected cells via the Fas/Fas ligand or the granzyme/perforin systems. The corticotropin-releasing hormone (CRH) is an important regulator for the body's stress response. It promotes proliferation and migration of various cancer cells through the CRH type 1 receptor under stress, and also inhibits NK or T cell activity. However, the relationship of CRH and NK cell migration to the target has not been confirmed. Herein, we study the effect of CRH on NK cell migration.

Methods

We used the human NK cell line, NK-92MI, and tested the expression of CRH receptor type 1 on NK-92MI by RT-PCR. This was to examine the effect of CRH on tumor and NK cell migration, thus NK cells (NK-92MI) were incubated with or without CRH and then each CRH treated cell's migration ability compared to that of the CRH untreated group.

Results

We confirmed that CRH receptor type 1 is expressed in NK-92MI. CRH can decrease NK cell migration in a time-/dose-dependent manner.

Conclusion

These data suggest CRH can inhibit NK cell migration to target cells.

Keywords: Corticotropin releasing hormone (CRH), NK cell, migration

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