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Kim and Joo: The Expression of Galectin-3, a Beta-Galactoside Binding Protein, in Dendritic Cells
Original Article

Immune Network 2005; 5(2): 105-109.

Published online: 30 June 2005

DOI: https://doi.org/10.4110/in.2005.5.2.105

The Expression of Galectin-3, a Beta-Galactoside Binding Protein, in Dendritic Cells

Mi-Hyoung Kim, Hong-Gu Joo

Department of Veterinary Medicine, Cheju National University, Jeju, Republic of Korea.

Correspondence to: Hong-Gu Joo, Department of Veterinary Medicine, Cheju National University, Ara 1-dong, Jeju 690-756, Republic of Korea. (Tel) 82-64-754-3379, (Fax) 82-64-756-3354, jooh@cheju.ac.kr

Copyright © 2005 The Korean Association of Immunologists

(open-access):

This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Dendritic cells (DCs) are the most potent APCs (antigen-presenting cells) and play a critical role in immune responses. Galectin-3 is a biological lectin with a beta-galactoside binding affinity. Recently, proteomic analysis revealed the presence of galectin-3 in the exosome of mature DCs. However, the expression and function of galectin-3 in DCs remains unclear yet.

Methods

We used bone marrow-derived DCs of mouse and showed the expression of galectin-3 in DCs by using flow cytometry analysis and Western blot analysis.

Results

Galectin-3 was determined as single band of 35 kDa in Western blot analysis. Flow cytometry analysis showed the major growth factor for DCs, granulocyte-macrophage colony stimulating factor (GM-CSF) and maturing agents, anti-CD40 monoclonal antibody (mAb) and lipopolysaccharide (LPS) consistently increased the intracellular expression of galectin-3 in DCs compared to medium alone. In addition, DCs treated with maturing agents did marginally express galectin-3 on their surface.

Conclusion

This study suggests that galectin-3 in DCs may be regulated by critical factors for DC function.

Keywords: Dendritic cells, galectin-3, viability, proliferaion

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