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Jung, Ahn, Do, Lemonnier, Tirosh, Tzehoval, Vadai, Eisenbach, and Do: Adoptive Transfer of Colon Cancer Derived Peptide-specific CD8+ T Cells in HHD Mice
Original Article

Immune Network 2004; 4(1): 31-37.

Published online: 31 March 2004

DOI: https://doi.org/10.4110/in.2004.4.1.31

Adoptive Transfer of Colon Cancer Derived Peptide-specific CD8+ T Cells in HHD Mice

Hun-Soon Jung1, In-Sook Ahn1, Hyung-Ki Do1, Francois A. Lemonnier2, Boaz Tirosh3, Esther Tzehoval3, Ezra Vadai3, Lea Eisenbach3, Myoung-Sool Do1

1School of Life and Food Sciences, Handong Global University, Pohang, Korea.

2AIDS-retrovirus Department, Antiviral Cellular Unit, Pasteur Institute, Paris, France.

3Department of Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel.

Corresponding Author (msdo@handong.edu)

Copyright © 2004 The Korean Association of Immunologists

(open-access):

This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

1-8D gene is a member of human 1-8 interferon inducible gene family and is shown to be overexpressed in fresh colon cancer tissues. Three peptides 1-6, 3-5 and 3-7 derived from 1-8D gene were shown to have immunogenicity against colon cancer.

Methods

To study tumor immunotherapy of these peptides we established an adoptive transfer model. Db-/-×β2 microglobulin (β2m) null mice transgenic for a chimeric HLA-A2.1/Db-β2m single chain (HHD mice) were immunized with irradiated peptide-loaded RMA-S/HHD/B7.1 transfectants. Spleens were removed after last immunization, and splenocytes were re-stimulated in vitro. Lymphocytes from vaccinated HHD mice were transferred together with IL-2 to the tumor bearing nude mice that were challenged S.C. with the HCT/HHD/B7 colon carcinoma cell line that was found to grow in these mice.

Results

Peptide 3-5 was found to be highly effective in CTL activity. Adoptively transferred anti-peptide 3-5 cytolytic T lymphocytes caused significant retardation in tumor growth.

Conclusion

This study shows that peptide 3-5 can be the most effective candidate for the vaccine of adoptive immunotherapy against colon cancer.

Keywords: 1-8D gene, HHD mice, colon caner, cytotoxic T lymphocyte, adoptive transfer

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