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Lim, Lee, Park, and Choi: IFN-γ Regulates Expression of BRG1 Associated Factor 155/170 and Sensitivity to Steroid in Astrocytes
Original Article

Immune Network 2004; 4(4): 224-228.

Published online: 31 December 2004

DOI: https://doi.org/10.4110/in.2004.4.4.224

IFN-γ Regulates Expression of BRG1 Associated Factor 155/170 and Sensitivity to Steroid in Astrocytes

Jung-Hee Lim1, Jeonggi Lee1, Joo Young Park2, In Hong Choi1

1Department of Microbiology and Institute for Immunology and Immunological Diseases, Yonsei University College of Medicine, Seoul, Korea.

2Department of Microbiology, Wonju College of Medicine, Yonsei University, Wonju, Korea.

Corresponding Author (inhong@yumc.yonsei.ac.kr)

Copyright © 2004 The Korean Association of Immunologists

(open-access):

This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

The expression of BRG1 associated factors (BAF) 155 and BAF 170 in response to IFN-γ or TNF-α was studied in astrocytoma cell lines and primary astrocytes. BAFs are complexed with BRG1 and are also associated with activated glucocorticoid for glucocorticoid trans-activation.

Methods

IFN-γ was pretreated for 18 hrs and cells were incubated with IL-1 or TNF-α for 72 hrs or 96 hrs with different concentrations of steroid. Cell death was measured by LDH assay. BAF expression was assayed by RT-PCR.

Results

IFN-γ increased cell death by dexamethasone in LN215 cells but not in LN319 cells. The IFN-γ increased the expression of BAF 155 and BAF 170 in adult astrocytes and LN215 cells, but IFN-γ decreased the expression of BAF 155/170 in LN319 cells. The effect of IFN-γ on the expression of BAF was not as clear in fetal astrocytes as it was in adult astrocytes.

Conclusion

Our results suggest cytokines produced during immune reaction or immunotherapy may modulate steroid susceptibility of astrocytes and astrocytoma cells by influencing the expression of BAFs.

Keywords: Astrocyte, steroid, BAF155/170, IFN-γ

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