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Yeo, Cho, Park, and Rothstein: B-1 Cells Differ from Conventional B (B-2) Cells: Difference in Proliferation
Original Article

Immune Network 2004; 4(3): 155-160.

Published online: 30 September 2004

DOI: https://doi.org/10.4110/in.2004.4.3.155

B-1 Cells Differ from Conventional B (B-2) Cells: Difference in Proliferation

Seung Geun Yeo1, Joong Saeng Cho1, Dong Choon Park2, Thomas L. Rothstein3

1Department of Otolaryngology, College of Medicine, Kyung Hee University, Seoul, Korea.

2Department of Obstetric and Gynecology, College of Medicine, Catholic University, Suwon, Korea.

3Department of Medicine, School of Medicine and Immunobiology Unit, Evans Memorial Department of Clinical Research, Medical Center, Boston University, USA.

Corresponding Author (dcpark@catholic.ac.kr)

Copyright © 2004 The Korean Association of Immunologists

(open-access):

This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

B-1 cells differ from conventional B-2 cells both phenotypically and functionally. The aim of this study was to investigate the difference between peritoneal B-1 cells and splenic B-2 cells in proliferation.

Methods

We obtained sorted B-1 cells from peritoneal fluid and B-2 cells from spleens of mice. During the culture of these cells, immunoglobulin secreted into the culture supernatants was evaluated by enzyme-linked immunosorbent assay. Entering of S phase in response to LPS-stimuli was measured by proliferative assay.

Results

Spontaneous Immunoglobulin M production occurred in peritoneal B-1 cells but not in splenic B-2 cells. LPS stimulated peritoneal B-1 cells secreted IgM at day 1, but splenic B-2 cells at day 2. In thymidine incorporation, peritoneal B-1 cells entered actively S phase after 24hours LPS-stimulation but splenic B-2 cells entered actively S phase after 48 hours.

Conclusion

IgM secretion and S phase entering occurred early in peritoneal B-1 cells compared to splenic B-2 cells.

Keywords: Peritoneal B-1 cell, splenic B-2 cell, immunoglobulin·proliferation

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