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Jin, Kim, and Park: Expression of Intracellular Single Chain Antibody Specific to Hepatitis B Virus X Protein
Original Article

Immune Network 2003; 3(1): 23-28.

Published online: 31 March 2003

DOI: https://doi.org/10.4110/in.2003.3.1.23

Expression of Intracellular Single Chain Antibody Specific to Hepatitis B Virus X Protein

Young Hee Jin, Hyung-Il Kim, Sun Park

Department of Microbiology, Ajou University School of Medicine, Suwon, Korea.

Corresponding author (sinsun@ajou.ac.kr)

Copyright © 2003 The Korean Association of Immunologists

(open-access):

This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Intracellular antibody specific to hepatitis B virus X protein (HBx) might be useful for studying the role of HBx in hepatocellular carcinogenesis and HBV replication.

Methods

With variable region genes for H7 monoclonal anti-HBx Ab, we constructed a vector for bacterial expression of single chain Ab (scFv) and a vector for eukaryotic cell expression of it. The expression of H7 scFv and its binding activity against HBx was examined by immunoblotting and immunofluorescence microscopy.

Results

H7 scFv expressed in bacterial cells retained reactivity to HBx. We demonstrated its intracytoplasmic expression in CosM6 eukaryotic cells.

Conclusion

This is the first study showing the expression of intracellular anti-HBx Ab in eukaryotic cells. H7 scFv may be a good tool to study the function of HBx in HBV infection.

Keywords: Intracellular Ab, hepatitis B virus, X protein

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