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Song, Nam, An, Lee, Kim, Park, Suhr, Jung, Na, Paik, and Jo: CD40-CD40 Ligand Interactions in the Production of IL-12 and IFN-γ by Tuberculous Pleural Mononuclear Cells
Original Article

Immune Network 2002; 2(3): 142-149.

Published online: 30 September 2002

DOI: https://doi.org/10.4110/in.2002.2.3.142

CD40-CD40 Ligand Interactions in the Production of IL-12 and IFN-γ by Tuberculous Pleural Mononuclear Cells

Chang-Hwa Song1, Hyun-Hee Nam1, Jeun-Ok An1, Ji-Sook Lee1, Hwa-Jung Kim1, Jeong-Kyu Park1, Ji-Won Suhr2, Sung-Soo Jung3, Moon-Jun Na4, Tae-Hyun Paik5, Eun-Kyeong Jo1

1Department of Microbiology, College of Medicine, Chungnam National University, Daejeon, Korea.

2Department of Internal Medicine, Catholic University, Daejeon, Korea.

3Department of Internal Medicine, College of Medicine, Chungnam National University, Daejeon, Korea.

4Department of Internal Medicine, College of Medicine, Konyang University, Chungnam, Korea.

5Department of Microbiology, College of Medicine, Konyang University, Chungnam, Korea.

Corresponding Author (hayoungj@cnu.ac.kr)

Copyright © 2002 The Korean Association of Immunologists

(open-access):

This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Our previous study showed that purified protein derivative (PPD)-stimulated pleural mononuclear cells (PMC) from tuberculous pleurisy (Tbp) produced significantly more IFN-γ (10- to 70-fold) after in vitro PPD stimulation than freshly isolated pleural cells from malignant pleurisy. The present study was designed to determine whether blocking the CD40-CD40 ligand (CD40L) interaction decreases IFN-γ production by altering IL-12 levels.

Methods

IL-12 and IFN-γ production after neutralizing anti-CD40L antibody treatment was compared to the efficacy of anti-CD80, anti-CD86, and a combination of anti-CD80 and CD86 (CD80+86) monoclonal antibodies (mAb). These activities were measured by enzyme-linked immunosorbent assays (ELISAs) and reverse transcription-polymerase chain reaction (RT-PCR), after in vitro stimulation with PPD antigen (Ag).

Results

Neutralization of CD80, CD86 and CD80+86 did not decrease IFN-γ and IL-12 production in Tbp-PMC, whereas neutralization of CD40L significantly depressed IL-12 p40 and IFN-γ. In addition, neutralization of CD40L completely inhibited IL-12 p40 and IFN-γ mRNA expression.

Conclusion

The CD40-CD40L interaction might play a major role in IL-12 and IFN-γ production in Tbp-PMC, thus contributing to protective immunity in human tuberculosis.

Keywords: CD40 ligand, tuberculous pleurisy, pleural mononuclear cells, CD80, CD86, PPD antigen, 30-kDa antigen

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