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Wolisi, Srirangam, Vinay, Suh, Suh, Choi, and Kwon: Alloimmune and Skin Allograft Responses in 4-1BB (CD137)-deficient Mice
Original Article

Immune Network 2002; 2(3): 133-136.

Published online: 30 September 2002

DOI: https://doi.org/10.4110/in.2002.2.3.133

Alloimmune and Skin Allograft Responses in 4-1BB (CD137)-deficient Mice

Godwin Wolisi1, Anjaiah Srirangam1, Dass S. Vinay2, Jae H. Suh3, Ho-Seok Suh4, Beom K. Choi5, Byoung S. Kwon2, 5

1Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN 48202, USA.

2LSU Eye Center, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA.

3Department of Diagnostic Pathology, Ulsan University Hospital, University of Ulsan, Korea.

4Department of Dermatology, Ulsan University Hospotal, University of Ulsan, Korea, Ulsan, Korea.

5The Immunomodulation Research Center and Department of Biological Sciences, University of Ulsan, Ulsan, Korea.

Corresponding Author (bskwon@mail.ulsan.ac.kr)

Copyright © 2002 The Korean Association of Immunologists

(open-access):

This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

The costimulatory molecule 4-1BB, a member of nerve growth factor receptor/tumor necrosis factor (NGFR/TNFR) super family, is involved in cell survival and death.

Methods

In this study, female C57BL/6 (H-2(b)) mice were used as a recipient, and DBA/2 (H-2(d)) as a donor to assess a mixed lymphocyte reaction (MLR) and CTL response in vitro, and skin graft survival. IL-2, IFN level was measured by ELISA.

Results

Mixed lymphocyte reaction (MLR) analysis showed that 4-1BB-deficient responder cells showed enhanced cellular proliferation over littermate controls. In contrast, IL-2 production was diminished only in 4-1BB knockout cultures. The IFN expression, on the other hand, was comparable between the groups. When female C57BL/6 (H-2(b)) mice were grafted with the trunk skin of DBA/2 (H-2d) mice, the in vivo tissue destruction of 4-1BB-deficient mice was not distinct from the normal littermates.

Conclusion

These data suggest that 4-1BB is critical for the induction of alloreactive responses in vitro but 4-1BB alone could not change the course of skin rejection in vivo.

Keywords: 4-1BB, skin graft, alloimmunity, MLR

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