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Yang, Seoh, and Jang: Regulatory Eosinophils in Inflammation and Metabolic Disorders

Abstract

Eosinophils are potent effector cells implicated in allergic responses and helminth infections. Responding to stimuli, they release their granule-derived cytotoxic proteins and are involved in inflammatory processes. However, under homeostatic conditions, eosinophils are abundantly present in the intestine and are constantly in contact with the gut microbiota and maintain the balance of immune responses without inflammation. This situation indicates that intestinal eosinophils have an anti-inflammatory function unlike allergic eosinophils. In support of this notion, some papers have shown that eosinophils have different phenotypes depending on the site of residence and are a heterogeneous cell population. Recently, it was reported that eosinophils in the small intestine and adipose tissue, respectively, contribute to homeostasis of intestinal immune responses and metabolism. Accordingly, in this review, we summarize new functions of eosinophils demonstrated in recent studies and discuss their homeostatic functions.

Abbreviations

EPO

eosinophil peroxidase

SIRPα

signal regulatory protein α

ILC2 cells

type 2 innate lymphoid cells

LP

lamina propria

PPs

Peyer's patches

TFH cells

follicular B helper T cells

IL-1Ra

IL-1 receptor antagonist

BAFF

B cell activating factor of the tumor-necrosis factor family

APRIL

proliferation-inducing ligand

DCs

dendritic cells

Th1

IFN-γ-producing CD4+ T cells

Th2

IL-4-producing CD4+ T cells

Th17

IL-17-producing CD4+ T cells

Treg cells

regulatory CD4+ T cells

M1 macrophages

classically activated macrophages

M2 macrophages

alternatively activated macrophages

UCP-1

uncoupling protein-1

INTRODUCTION

Eosinophils are potent effector cells implicated in allergic responses and helminth infections and have cytotoxic granules containing major basic protein (MBP), eosinophil cationic protein (ECP), eosinophil peroxidase (EPO), and eosinophil-derived neurotoxin (EDN) (1). Responding to stimuli such as an allergen, helminth infection, or tissue injury, they release their granule-derived cytotoxic proteins and are involved in inflammatory processes. However, the intestine (constantly in contact with the gut microbiota) is a major site where eosinophils are abundantly present under homeostatic conditions. Intestinal eosinophils rarely undergo degranulation and have a long lifespan as compared with eosinophils in the blood and lungs (23). A possible reason is that inhibitory receptor signal regulatory protein α (SIRPα)/CD172a, highly expressed on the intestinal eosinophils, inhibits degranulation of eosinophils and promotes their survival (2). In support of this notion, the ligand for SIRPα/CD172a, CD47, is expressed in the intestine (2). In addition, intestinal eosinophils highly express common γ-chain, which is an integral part of the cytokine receptors for IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21; intestinal eosinophils are severely reduced in number in the common γ-chain-deficient mice unlike eosinophils in blood and lungs; therefore, common γ-chain-dependent signals seem to play an important role in selective survival of intestinal eosinophils (3). Intestinal eosinophils are produced from hematopoietic stem cells in bone marrow and migrate into the intestine under the influence of chemokine eotaxin-1 (14). During the development and survival of eosinophils, signaling of the common β-receptor chain shared by IL-3, IL-5, and GM-CSF is required (4), and among these cytokines, IL-5 is particularly important; overproduction of IL-5 in transgenic mice results in eosinophilia (5) whereas deletion of IL-5 abrogates eosinophilia induced by an aeroallergen (6). According to a recent report, IL-5 is mainly produced by type 2 innate lymphoid (ILC2) cells, and its production in ILC2 cells is enhanced by vasoactive intestinal peptide (7). Additionally, transcription factor GATA-1 is crucial for the development of eosinophils; deletion of the palindromic double GATA high-affinity binding sites in the GATA-1 promoter results in eosinophil-deficient mice (called Δdbl GATA-1 mice) (8). PHIL mice, which express diphtheria toxin A chain under the control of the eosinophil-specific EPO promoter, are also eosinophil-deficient mice (9). In recent studies on these eosinophil-deficient mice, it has been revealed why eosinophils are abundant in the intestine without inflammation and what their physiological roles are under homeostatic conditions. Some papers have shown that intestinal eosinophils promote formation and maintenance of IgA-expressing plasma cells (10) and abundantly produce IL-1 receptor antagonist (IL-1Ra) (11), contributing to intestinal immune homeostasis. Several lines of evidence have shown that eosinophils also exist in adipose tissue and are involved in metabolic homeostasis (1213). Therefore, in this review, we summarize recent findings regarding eosinophils and discuss the homeostatic functions of these cells.

THE ANTI-INFLAMMATORY fUNCTION

Recently, it has been reported that small-intestinal eosinophils produce large amounts of IL-1Ra (11). The latter competes with IL-1β for receptor binding and inhibits inflammatory responses (14). Meanwhile, IL-1β is primarily produced by activated macrophages and functions as a key mediator in various inflammatory diseases including inflammatory bowel disease and rheumatoid arthritis (14). In this context, a decrease in the IL-1Ra to IL-1 ratio is linked to inflammatory bowel disease in humans (15). IL-1Ra-deficient mice spontaneously develop arthritis with a marked increase in the number of Th17 cells (1617). In line with this finding, it has been reported that microbiota-induced IL-1β, but not IL-6, plays an important role in the development of Th17 cells in the intestine (18). Therefore, small-intestinal eosinophils suppress the differentiation and maintenance of Th17 cells by constantly secreting large amounts of IL-1Ra, thus contributing to intestinal immune homeostasis (11) (Fig. 1). Intestinal eosinophils also inhibit differentiation and/or proliferation of Th1 cells in the lamina propria (LP) though less strongly in comparison with the effects on Th17 cells (11). However, because IL-1Ra-deficient mice do not show a significant increase in the number of Th1 cells, intestinal eosinophils may suppress differentiation and/or proliferation of Th1 cells by secreting other unidentified cytokines. Unlike Th1 and Th17 cells, small-intestinal eosinophils do not affect frequencies of Foxp3+ regulatory T (Treg) cells and Th2 cells in the LP (11). Particularly in food allergy, intestinal eosinophils activate dendritic cells (DCs) through a release of EPO and promote their migration from the intestine to draining lymph nodes, thus initiating a primary Th2 immune response (19). In contradiction to these findings, one group reported that the numbers of CD103+ DCs and CD103+ CD4+ T cells including Foxp3+ Treg cells are reduced in the LP of eosinophil-deficient mice, although they did not explain the mechanism in the paper (10), and this phenomenon should be confirmed later.
Because small-intestinal eosinophils, but not eosinophils in blood and bone marrow, produce a large amount of IL-1Ra under homeostatic conditions, we can hypothesize that gut microbiota and its metabolites may be involved in IL-1Ra production. On the other hand, small-intestinal eosinophils in both SPF and GF mice produce similar levels of IL-1Ra, and metabolites such as retinoic acid and short-chain fatty acids do not induce IL-1Ra production in bone marrow eosinophils (11). Instead, GM-CSF, which is known to be involved in the development and survival of eosinophils, performs an important function in IL-1Ra production (11). In this regard, the high basal concentration of GM-CSF in the small intestine and high expression of GM-CSF receptor in small-intestinal eosinophils seem to explain the unique ability of small-intestinal eosinophils to continuously secrete a large amount of IL-1Ra under steady-state conditions. In addition, because Th17 cells can produce GM-CSF in an IL-1- and IL-23-dependent manner (20), it is plausible that GM-CSF produced by Th17 cells endows eosinophils with the superior capacity for IL-1Ra production and regulates Th17 cell homeostasis via a negative feedback loop. In contrast, ironically, large-intestinal eosinophils, when activated by GM-CSF produced by Th17 cells, secret EPO as well as proinflammatory cytokines TNF and IL-13, and aggravate colitis (21). These findings imply that small-intestinal eosinophils are different from large-intestinal ones. In support of this notion, large-intestinal eosinophils produce much smaller amounts of IL-1Ra and show a distinctive expression pattern of surface proteins including CD11c, ST2, and Ly6C as compared with small-intestinal ones (19). In addition, although eosinophils are mainly present throughout the intestine, large-intestinal eosinophils are scarce in a steady state (1119), and only under intestinal inflammatory conditions, does their frequency dramatically increase (21). Accordingly, large-intestinal eosinophils seem to be proinflammatory unlike their small-intestinal counterparts.

ANTIBACTERIAL DEFENSE

Most of the intestinal plasma cells are located in the LP and mostly produce IgA (22). IgA is secreted into the intestinal lumen and functions as the first line of defense through neutralization and clearance of enteric pathogens. Nevertheless, IgA-producing B cells are generally formed in Peyer's patches (PPs) and migrate into the LP (22). In PPs, antigen-experienced CD4+ T cells migrate into B-cell follicles via CXCR5 and activate B cells (22). These specialized CD4+ T cells are called follicular B helper T (TFH) cells and are different from other CD4+ T cell lineages. They highly express costimulatory molecules for their development and function, e.g., CD40L, OX-40, and programmed cell death 1 (PD-1) rather than cytokines and transcription factors characteristic of Th1, Th2, and Th17 cells (22). Transcription factor B cell lymphoma 6 (Bcl-6) plays an important role in TFH differentiation, whereas transcription factor B lymphocyte maturation protein 1 (Blimp-1) inhibits it (22). The activated B cells induce activation-induced cytidine deaminase (AID) and provoke class switch recombination (22). Because TGF-β1, a key cytokine for IgA switching, is expressed by many cells in PPs, activated B cells preferentially switch their isotype from IgM to IgA (2223). Moreover, IL-21 secreted by TFH cells enhances the proliferation and differentiation of IgA plasma cell precursors in synergy with TGF-β1 (24). Meanwhile, IgM+ B cells in the LP are activated by polyclonal stimuli or by antigens presented by DCs in the LP (LP-DCs) and differentiate into IgA-producing plasma cells without the help of TFH cells (22). In this T cell-independent formation of IgA-producing B cells, important functions are performed by TGF-β1, B-cell activating factor of the tumor-necrosis factor family (BAFF), and a proliferation-inducing ligand (APRIL) produced by DCs and stromal cells in the LP (22). In vitro, cytokines such as IL-6, TNF, IL-10, IL-4, and IL-5, also prolong the survival of plasma cells (25). In this context, bone marrow eosinophils have been reported to support the survival of plasma cells by secreting APRIL and IL-6 and contribute to the long-term maintenance of plasma cells in bone marrow (26). Although intestinal eosinophils are mainly in the LP, not in PPs, they are involved in the formation and maintenance of IgA-producing B cells in PPs as well as in the LP (10). Intestinal eosinophils express BAFF, TGF-β1, and matrix metalloproteases (that are involved in the release of TGF-β1 from the large latent complex) as well as APRIL and IL-6 at the mRNA level (10). Therefore, intestinal eosinophils appear to influence T-cell-independent formation and maintenance of IgA-producing B cells via production of these cytokines and enzymes. Accordingly, the amount of IgA is dramatically reduced in the intestine of eosinophil-deficient mice; this change increases bacterial load in the gut and affects composition of the microbiota there (10). Therefore, it seems likely that eosinophils indirectly exert their effects on T-cell-dependent formation of IgA-producing B cells in PPs. Recently, one paper revealed that eosinophils can be involved in IgA production through IL-1β (27). Moreover, eosinophils can release mitochondrial DNA in a catapult-like manner and kill bacteria (28). Nevertheless, the release of mitochondrial DNA does not cause cell death of eosinophils. For the release of mitochondrial DNA, eosinophils should be activated by lipopolysaccharide under conditions where they are primed with IL-5 or IFN-γ (28). In the intestinal environment, gut bacteria can exert their effects on immune cells through bacteria-derived molecules, and immune-cell populations producing IFN-γ (Th1) and IL-5 (ILC2) exist; therefore, it seems plausible that intestinal eosinophils reduce the bacterial load in the gut via the release of mitochondrial DNA under homeostatic conditions. Accordingly, intestinal eosinophils prevent the host from bacterial invasion and contribute to homeostasis of intestinal immunity.

METABOLIC HOMEOSTASIS

Obesity induces chronic low-grade inflammation, consequently causing insulin resistance and type 2 diabetes (2930). In the obese state, a large number of monocytes migrate into adipose tissue and differentiate into classically activated (M1) macrophages expressing TNF-α and inducible nitric oxide synthase, which triggers inflammation and results in metabolic diseases (2930). By contrast, resident adipose-tissue macrophages in the lean state express IL-10 and arginase 1, and show an anti-inflammatory phenotype of alternatively activated (M2) macrophages (2930). Activation of the anti-inflammatory M2 macrophages requires Th2 cytokines such as IL-4 and IL-13 (Fig. 1), whereas activation of M1 macrophages requires Th1 cytokine IFN-γ (2930). Eosinophils are a major IL-4-expressing cell population in adipose tissue and help to sustain M2 macrophages (12) (Fig. 1). High-fat diet-induced obesity significantly reduces the number of eosinophils in adipose tissue (12). Therefore, eosinophil-deficient mice show aggravation of obesity-induced metabolic diseases via a dramatic reduction in the number of M2 macrophages in adipose tissue, indicating that eosinophils are intimately involved in metabolic homeostasis (12). Besides, ILC2 cells promote accumulation of eosinophils and activate M2 macrophages in adipose tissue through production of IL-5 and IL-13 and are implicated in metabolic homeostasis (3132).
Eosinophils are also involved in the biogenesis of beige fat (1333). The latter represents clusters of uncoupling protein 1 (UCP-1)-expressing adipocytes in white adipose tissue and functions in heat production under conditions of increased energy expenditure, such as cooling and exercise (3435). Thus, beige adipocytes contribute to suppression of obesity and alleviation of metabolic diseases. By contrast, white adipocytes do not express UCP-1 and are not involved in thermogenesis; instead, they participate in fat storage, contributing to obesity-induced metabolic diseases (35). IL-4 released by eosinophils induces the tyrosine hydroxylase expression of M2 macrophages under cold conditions and drives these macrophages to produce catecholamines such as norepinephrine (13). The macrophage-derived catecholamines trigger expression of the UCP-1 gene in subcutaneous white adipose tissue and are involved in the development of beige fat (13). In addition, meteorinlike (Metrnl), which is a hormone induced in muscle after exercise and in adipose tissue upon cold exposure, exerts its effect on eosinophils and increases their expression levels of Th2 cytokines such as IL-4 and IL-13, consequently promoting the development of (and thermogenesis in) beige fat (36). In particular, ILC2 cells contribute to the biogenesis of beige fat through production of methionine-enkephalin peptide as well as via activation by IL-33 receptor signaling (32).
Increasing evidence suggests that obesity and other metabolic syndrome are associated with altered composition of the gut microbiota (37). In agreement with this notion, the transfer of gut microbiota from obese to germ-free mice increases obesity more than gut microbiota from lean mice does (38). Microbiota depletion either by antibiotic treatment or in germ-free mice promotes beige fat biogenesis and alleviates obesity and other metabolic syndrome; these effects are mediated by eosinophil accumulation, enhanced Th2 cytokine signaling, and M2 macrophage polarization in white adipose tissue of microbiota-depleted mice (39). In this regard, intestinal eosinophils may inhibit obesity and other metabolic syndrome through a reduction of bacterial burden via IgA production (1027). In addition, because obesity-induced metabolic diseases show elevated levels of IL-1β (29), intestinal eosinophils may alleviate inflammation by producing large amounts of IL-1Ra and thus alleviate metabolic diseases.

PROSPECTS

According to recent reports, eosinophils are a heterogeneous cell population and have different characteristics depending on the site of residence (3111921). Although eosinophils are mainly distributed in the intestine, the phenotype of large-intestinal eosinophils is different from that of small-intestinal ones, and the number of large-intestinal eosinophils in a steady state is much smaller than that of their small-intestinal counterparts (111921). Moreover, small-intestinal eosinophils in response to GM-CSF produce large amounts of IL-1Ra and exert an anti-inflammatory function (11), whereas large-intestinal eosinophils increase production of proinflammatory cytokines TNF-α and IL-13 and promote colitis (21). These observations mean that eosinophils do not all have an identical function, but rather their function is likely controlled by local milieu. Thus, if anti-inflammatory eosinophils can be upregulated or inflammatory eosinophils can be converted into anti-inflammatory ones, then these modalities should help to combat inflammatory diseases such as allergies, inflammatory bowel disease, and obesity-related metabolic diseases. To this end, it would be worthwhile to identify the surface markers that can distinguish homeostatic and inflammatory eosinophils. The extract of Ulmus davidiana var. japonica Nakai increases the number of small-intestinal eosinophils and suppresses differentiation and/or proliferation of Th1 and Th17 cells (40). Regulatory eosinophil-recruiting prebiotics or probiotics may be useful for prevention of inflammatory and metabolic diseases. More studies should be conducted in this field for successful development of eosinophil-targeting treatments.

Figures and Tables

Figure 1

Anti-inflammatory functions of eosinophils. Small-intestinal eosinophils suppress differentiation and/or proliferation of Th17 cells via production of a large amount of IL-1Ra and are involved in homeostasis of intestinal immunity. Meanwhile, eosinophils in adipose tissue activate M2 macrophages through IL-4 expression and inhibit inflammation, thereby contributing to metabolic homeostasis.

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Abbreviations

EPO

eosinophil peroxidase

SIRPα

signal regulatory protein α

ILC2 cells

type 2 innate lymphoid cells

LP

lamina propria

PPs

Peyer's patches

TFH cells

follicular B helper T cells

IL-1Ra

IL-1 receptor antagonist

BAFF

B cell activating factor of the tumor-necrosis factor family

APRIL

proliferation-inducing ligand

DCs

dendritic cells

Th1

IFN-γ-producing CD4+ T cells

Th2

IL-4-producing CD4+ T cells

Th17

IL-17-producing CD4+ T cells

Treg cells

regulatory CD4+ T cells

M1 macrophages

classically activated macrophages

M2 macrophages

alternatively activated macrophages

UCP-1

uncoupling protein-1

ACKNOWLEDGEMENTS

This work was supported by the Ministry of Education, Culture, Sports, Science and Technology of Japan (18790338 and 19041044), Institute for Basic Science (IBS) project (IBS-R005-D1-2015-a00) and the National Research Foundation of Korea (NRF) (NRF-2015M3A 9B6073846).

References

1. Rothenberg ME, Hogan SP. The Eosinophil. Annu Rev Immunol. 2006; 24:147–174.
crossref
2. Garcia NV, Umemoto E, Sito Y, Yamasaki M, Hata E, Matozaki T, Murakami M, Jung YJ, Woo SY, Seoh JY, Jang MH, Aozasa K, Miyasaka M. SIRPα/CD172a regulates eosinophil homeostasis. J Immunol. 2011; 187:2268–2277.
crossref
3. Carlens J, Wahl B, Ballmaier M, Bulfone-Paus S, Förster R, Pabst O. Common γ-chain-dependent signal confer selevtive survival of eosinophils in the murine small intestine. J Immunol. 2009; 183:5600–5607.
crossref
4. Mishra A, Hogan SP, Lee JJ, Foster PS, Rothenberg ME. Fundamental signals that regulate eosinophil homing to the gastrointestinal tract. J Clin Invest. 1999; 103:1719–1727.
crossref
5. Dent LA, Strath M, Mellor AL, Sanderson CJ. Eosinophilia in transgenic mice expressing interleukin 5. J Exp Med. 1990; 172:1425–1431.
crossref
6. Foster PS, Hogan SP, Ramsay AJ, Matthaei KI, Young IG. Interleukin 5 deficiency abolishes eosinophilia, airways hyperreactivity, lung damage in a mouse asthma model. J Exp Med. 1996; 183:195–201.
crossref
7. Nussbaum JC, Dyken SJV, Moltke JV, Cheng LE, Mohapatra A, Molofsky AB, Thornton EE, Krummel MF, Chawla A, Liang HE, Locksley RM. Type 2 innate lymphoid cells control eosinophil homeostasis. Nature. 2013; 502:245–248.
crossref
8. Yu C, Cantor AB, Yang H, Browne C, Wells RA, Fujiwara Y, Orkin SH. Targeted deletion of a high-affinity GATA-binding site in the GATA-1 promoter leads to selective loss of the eosinophil lineage in vivo. J Exp Med. 2002; 195:1387–1395.
crossref
9. Lee JJ, Dimina D, Macias MP, Ochkur SI, Mcgarry MP, O'Neill KR, Protheroe C, Pero R, Nguyen T, Cormier SA, Lenkiewicz E, Colbert D, Rinaldi L, Ackerman SJ, Irvin CG, Lee NA. Defining a link with asthma in mice congenitally deficient in eosinophils. Science. 2004; 305:1773–1776.
crossref
10. Chu VT, Beller A, Rausch S, Strandmark J, Zänker M, Arbach O, Kruglov A, Berek C. Eosinophils promote generation and maintenance of immunoglobulin-A-expressing plasma cells and contribute to gut immune homeostasis. Immunity. 2014; 40:582–593.
crossref
11. Sugawara R, Lee EJ, Jang MS, Jeun EJ, Hong CP, Kim JH, Park A, Yun CH, Hong SW, Kim YM, Seoh JY, Jung Y, Surh CD, Miyasaka M, Yang BG, Jang MH. Small intestinal eosinophils regulate Th17 cells by producing Il-1 receptor antagonist. J Exp Med. 2016; 213:555–567.
crossref
12. Wu D, Molofsky AB, Liang HE, Ricardo-Gonzalez RR, Jouihan HA, Bando JK, Chawla A, Locksley RM. Eosinophils sustain adipose alternatively activated macrophages associated with glucose homeostasis. Science. 2011; 332:243–247.
crossref
13. Qiu Y, Nguyen KD, Odegaard JI, Cui X, Tian X, Locksley RM, Palmiter RD, Chawla A. Eosinophils and type 2 cyotkine signaling in macrophages orchestrate development of functional beige fat. Cell. 2014; 157:1292–1308.
crossref
14. Slims JE, Smith DE. The IL-1 family: regulators of immunity. Nat Rev Immunol. 2010; 10:89–102.
crossref
15. Casini-Raggi V, Kam L, Chong YJ, Fiocchi C, Pizarro TT, Cominelli F. Mucosal imbalance of IL-1 and IL-1 receptor antagonist in inflammatory bowel disease. A novel mechanism of chronic intestinal inflammation. J Immunol. 1995; 154:2434–2440.
16. Nakae S, Saijo S, Horai R, Sudo K, Mori S, Kwakura Y. IL-17 production from activated T cells is required for the spontaneous development of destructive arthritis in mice deficient in IL-1 receptor antagonist. Proc Natl Acad Sci U S A. 2003; 100:5986–5990.
crossref
17. Koenders MI, Devesa I, Marijnissen RJ, Abdollahi-Roodsaz S, Boots AM, Walgreen B, di Padova FE, Nicklin MJ, Joosten LA, van den Berg WB. Interleukin-1 drives pathogenic Th17 cells during spontaneous arthritis in interleukin-1 receptor antagonist-deficient mice. Arthritis Rheum. 2008; 58:3461–3470.
crossref
18. Shaw M, Kamada N, Kim YG, Núñez G. Microbiota-induced IL-1β, but not IL-6, is critical for the development of steady-state TH17 cells in the intestine. J Exp Med. 2012; 209:251–258.
crossref
19. Chu DK, Jimenez-Saiz R, Verschoor CP, Walker TD, Goncharova S, Llop-Guevara A, Shen P, Gordon ME, Barra NG, Bassett JD, Kong J, Fattouh R, McCoy KD, Bowdish DM, Erjefält JS, Pabst O, Humbles AA, Kolbeck R, Waserman S, Jordana M. Indigenous enteric eosinophils contraol DCs to initiate a primary Th2 immune response in vivo. J Exp Med. 2014; 211:1657–1672.
crossref
20. El-Behi M, Ciric B, Dai H, Yan Y, Cullimore M, Safavi F, Zhang GX, Dittel BN, Rostami A. The encephalitogenicity of TH17 cells is dependent on IL-1- and IL-23-induced production of the cytokine GM-CSF. Nat Immunol. 2011; 12:568–575.
crossref
21. Griseri T, Arnold IC, Pearson C, Krausgruber T, Schiering C, Franchini F, Schulthess J, McKenzie BS, Crocker PR, Powrie F. Granulocyte macrophage colony-stimulating factor-activated eosinophils promote interleukin-23 drive chronic colitis. Immunity. 2015; 43:187–199.
crossref
22. Fagarasan S, Kawamoto S, Kanagawa O, Suzuki K. Adaptive immune regulation in the gut: T cell-dependent and T cell-independent IgA synthesis. Annu Rev Immunol. 2010; 28:243–273.
crossref
23. Cazac BB, Roes J. TGF-β receptor controls B cell responsiveness and induction of IgA in vivo. Immunity. 2000; 13:443–451.
crossref
24. Dullaers M, Li D, Xue Y, Ni L, Gayet I, Morita R, Ueno H, Palucka KA, Banchereau J, Oh S. A T cell-dependent mechanism for the induction of human mucosal homing immunoglobulin A-secreting plasmablasts. Immunity. 2009; 30:120–129.
crossref
25. Cassese G, Arce S, Hauser AE, Lehnert K, Moewes B, Mostarac M, Muehlinghaus G, Szyska M, Radbruch A, Manz RA. Plasma cell survival is mediated by synergistic effects of cytokines and adhesion-dependent signals. J Immunol. 2003; 171:1684–1690.
crossref
26. Chu VT, Fröhlich A, Steinhauser G, Scheel T, Roch T, Fillatreau S, Lee JJ, Löhning M, Berek C. Eosinophils are required for the maintenance of plasma cells in the bone marrow. Nat Immunol. 2011; 12:151–159.
crossref
27. Jung Y, Wen T, Mingler MK, Caldwell JM, Wang YH, Chaplin DD, Lee EH, Jang MH, Woo SY, Seoh JY, Miyasaka M, Rothenberg ME. IL-1β in eosinophil-mediated small intestinal homeostasis and IgA production. Mucosal Immunol. 2015; 8:930–942.
crossref
28. Yousefi S, Gold JA, Andina N, Lee JJ, Kelly AM, Kozlowski E, Schmid I, Straumann A, Reichenbach J, Gleich GJ, Simon HU. Catapult-like release of mitochondrial DNA by eosinophils contributes to antibacterial defense. Nat Med. 2008; 14:949–953.
crossref
29. Donath MY, Shoelson SE. Type 2 diabetes as an inflammatory disease. Nat Rev Immunol. 2011; 11:98–107.
crossref
30. Lackey DE, Olefsky JM. Regulation of metabolism by the innate immune system. Nat Rev Endocrinol. 2016; 12:15–28.
crossref
31. Molofsky AB, Nussbaum JC, Liang HE, Dyken SJV, Cheng LE, Mohapatra A, Chawla A, Lacksley RM. Innate lymphoid type 2 cells sustain visceral adipose tissue eosinophils and alternatively activated macrophages. J Exp Med. 2013; 210:535–549.
crossref
32. Brestoff JR, Kim BS, Saenz SA, Stine RR, Monticelli LA, Sonnenberg GF, Thome JJ, Farber DL, Lutfy K, Seale P, Artis D. Group 2 innate lymphoid cells promote beiging of white adipose tissue and limit obesity. Nature. 2015; 519:242–246.
crossref
33. Lee SD, Tontonoz P. Eosinophils in fat: pink is the new brown. Cell. 2014; 157:1249–1250.
crossref
34. Harms M, Seale P. Brown and beige fat: development, function and therapeutic potential. Nat Med. 2013; 19:1252–1263.
crossref
35. Bartelt A, Heeren J. Adipose tissue browning and metabolic health. Nat Rev Endocrinol. 2014; 10:24–36.
crossref
36. Rao RR, Long JZ, White JP, Svensson KJ, Lou J, Lokurkar I, Jedrychowski MP, Ruas JL, Wrann CD, Lo JC, Camera DM, Lachery J, Gygi S, Seehra J, Hawley JA, Spiegelman BM. Meteorin-like is a hormone that regulates immune-adipose interactions to increase beige fat thermogenesis. Cell. 2014; 157:1279–1291.
crossref
37. Winer DA, Luck H, Tsai S, Winer S. The intestinal immune system in obesity and insulin resistance. Cell Metab. 2016; 23:413–426.
crossref
38. Turnbaugh PJ, Ley RE, Mahowald MA, Magrini V, Mardis ER, Gordon JI. An obesity-associated gut microbiome with increased capacity for energy harvest. Nature. 2006; 444:1027–1031.
crossref
39. Suárez-Zamorano N, Fabbiano S, Chevalier C, Stojanović O, Colin DJ, Stevanović A, Veyrat-Durebex C, Tarallo V, Rigo D, Germain S, Ilievska M, Montet X, Seimbille Y, Hapfelmeier S, Trajkovski M. Microbiota depletion promotes browning of white adipose tissue and reduces obesity. Nat Med. 2015; 21:1497–1501.
crossref
40. Lee HS, Jang MS, Kim JH, Hong CP, Lee EJ, Jeun EJ, Kim C, Kim EK, Ahn KS, Yang BG, Ahn KS, Jang YP, Ahn KS, Kim YM, Jang MH. Ulmus davidiana var. japonica Nakai upregulates eosinophils and suppresses Th1 and Th17 cells in the small intestine. PLoS One. 2013; 8:e76716.
crossref
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