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Lee, Lee, Park, Nam, Shin, and Choe: 3C8, a new monoclonal antibody directed against a follicular dendritic cell line, HK
Original Article

Immune Network 2001; 1(1): 26-31.

Published online: 30 April 2001

DOI: https://doi.org/10.4110/in.2001.1.1.26

3C8, a new monoclonal antibody directed against a follicular dendritic cell line, HK

In Yong Lee1, Joonhee Lee1, Weon Seo Park2, Eui-Cheol Nam3, Yung Oh Shin1, Jongseon Choe1

1Department of Microbiology, Kangwon National University College of Medicine, Chunchon, Kangwon, Korea.

2Department of Pathology, Kangwon National University College of Medicine, Chunchon, Kangwon, Korea.

3Department of Otorhinolaryngology, Kangwon National University College of Medicine, Chunchon, Kangwon, Korea.

Correspondence: Jongseon Choe, Department of Microbiology, Kangwon National University College of Medicine, Chunchon, Kangwon 200-701 Korea. Tel: 033-250-8862, Fax: 033-242-7571, jchoe@kangwon.ac.kr

Copyright © 2001 The Korean Association of Immunologists

(open-access):

This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Follicular dendritic cells (FDCs) play key roles during T cell-dependent humoral immune responses by allowing antigen-specific B cells to survive, proliferate, and differentiate within the FDC networks of secondary follicles, i.e., germinal centers (GC).

Methods

A novel monoclonal antibody, 3C8, was generated by immunizing with an FDC line HK, in order to understand the molecular signals involved in the FDC-B cell interactions in the microenvironment of the GC.

Results

The 3C8 antibody did not bind to mononuclear cells, including T cells, B cells, and monocytes. Murine L929 and human skin fibroblasts exhibited no or little reactivity to 3C8. However, 3C8 specifically recognized HK cells by flowcytometry . Furthermore, the antigen recognized by 3C8 was restricted to the GC of the human tonsil. Dendritic networks of the GC were intensely stained by 3C8, but cells outside the GC were not .

Conclusion

Our results suggest that the antigen 3C8 may play some unique role on FDCs during the GC reactions.

Keywords: follicular dendritic cell, monoclonal antibody, germinal center

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