Abstract
Background
Korean mistletoe (Viscum album) extract has been found to posses immunostimulatory activity. In this study, Korean mistletoe extract, M11C (non-lectin components), was used to know whether this extract might activate mouse peritoneal macrophages to produce interleukin 1β(IL-1β).
Methods
Hemagglutination assay was carried out to examine whether M11C contained a lectin or not. To know the effect of M11C on the production of IL-1β, the macrophages were treated by the M11C, and then collected the supernatant (M11C stimulated macrophages-conditioned media; MMCM). MMCM was analyzed for the IL-1β quantification and mRNA expression by means of ELISA and RT-PCR, respectively.
Results
Maximum effective dose and time of M11C on IL-1β production from macrophages were 20µg/ml and 8 hours, respectively. This ELISA data was reconfirmed by immunoblotting assay. indicating that M11C is a good candidate for an immunomodulator. The dose and time dependent effects of M11C on the expression of IL-1β mRNA from macrophages was also shown in expression of mRNA detected by RT-PCR. Treatment dose and time for the maximum expression of IL-1β mRNA were 20 µg/ml and 4 hours, respectively. Maximum gene expression of IL-1β was much earlier than maximum production of it.