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Abstract
Background
The CBF/NF-Y enhancer region of ABO gene reported to contain 43bp minisatellite tandem repeats has been rarely reported. We describe here the relationship between minisatellite tandem repeats and ABO alleles in samples from Korean population with common ABO blood group and rare ABO subgroup.
Methods
Sixty one cases of ABO subgroup (14 A2, 12 A2B, 1 Aweak, 7 AweakB, 11 B3, 5 A1B3, 1 A1Bweak, 2 Bweak, and 8 cis-AB) and 41 cases of common ABO blood group (13 A, 6 AB, 11 B, and 11 O) were obtained from healthy donors at the Gwangju-Chonnam Red Cross Blood Center between Sep 2004 and Aug 2005. Red cells were phenotyped by standard serologic tests and genotyped by direct DNA sequencing exon 6 and 7 of the ABO gene. The minisatellite repeats were analyzed by PCR method.
Results
The ABO∗A101 and ∗A102 had only one repeat, ∗B101, ∗O01 and ∗O02 had 4 repeats in common ABO blood group, while the ∗A102, ∗cis-AB01, and ∗Aw10 had only one minisatellite repeat and ∗A201, ∗A204, ∗B101, ∗Bw03, ∗B306, ∗O01, and ∗O02 alleles had 4 repeats and unexpectedly 3 A2 cases with ∗A102 had 4 repeats in the rare ABO subgroup.
References
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Fig. 1.
(A) Structure of the CBF/ NF-Y enhancer region (rectangle with shading patterns) of ABO genes and primers Pro-F (5'GG AAACAAATCCTACCCCTAC3') and Pro-R (5'GTGCTGCCTGTGC CTGTTAC3') used in this study. (B) PCR products from representative ABO subgroups and their genotypes separated in 1.8% agarose gel after PCR amplification with primers Pro-F and Pro-R. Lane, M. molecular size marker; 1. A2 (A101/O02); 2. A2 (A201/O01); 3. A2B (A102/B101); 4. A2B (A204/B101); 5. AwB (Aw10/B101); 6. B3 (B101/O01); 7. A1B3 (A102/B101); 8. A1B3 (cis-AB01/A102); 9. A2B (A102/ B306); 10. A2B3 (cis-AB01/B101); 11. B3 (cis-AB01/O01).
Table 1.
Number of minisatellite repeats present in 102 samples from Korean blood donors with different ABO phenotypes and genotypes
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