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Abstract
The blast phase in chronic myelogenous leukemia (CML) is associated with mutation of several genes. It is well known that p53 gene mutation plays a key role in the myeloid or lymphoid blast phase of CML. But for the case of the N-ras gene, the association between N-ras mutations and the blast phase of CML is not yet known. We report here on a case of detecting N-ras point mutation without p53 mutation in a 64 year-old man who suffered from the lymphoblastic blast phase of CML.
References
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Fig. 1.
PCR-SSCP analysis of N-ras exon 1 in a patient with CML-blast phase. Band shift due to point mutation was detected on the arrowed sample. Controls included DNAs from a sample with known N-ras exon 1 mutation as a positive control (P), from normal lymphocytes as a normal control (W), and a negative control (no template DNA) (N).
Fig. 2.
Dinucleotide sequencing of exon 1 of N-ras gene from DNA of a patient with CML-blast phase. Sequences are shown 5' (top) to 3' (bottom). A codon 13, GGT, was mutated to CGT, substituting an aminoacid arginine for glycine. Arrow indicates the base that has the point mutation.
Fig. 3.
PCR-SSCP analysis of p53 gene of exon 5 (A), 6 (B), 7 (C), and 8 (D) in a patient with CML-blast phase. No band shift was observed in the patient (P).
Table 1.
Lymphoma-leukemia marker study
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