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Abstract
T-cell prolymphocytic leukemia (T-PLL) is a rare mature post-thymic T-cell malignancy with infiltration to the blood, bone marrow, lymph node, liver, spleen and skin; this disease has a poor prognosis and an aggressive clinical course. We report here on a case of CD56+ T-PLL that was diagnosed by hematological examination, immunophenotyping and molecular studies including determining the TCL1 expression by using reverse-transcriptase polymerase chain reaction (RT-PCR), and direct sequencing of the RT-PCR product.
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Fig. 1
Morphology of T-PLL cells. Peripheral blood (A) and bone marrow aspirate (B) show many small variants of prolymphocytes (Wright stain, ×1000). Bone marrow section (C) shows infiltration of monotonous leukemic cells and hypercellular marrow for age (50%) (H-E stain, ×100).
Fig. 2
Expression of the TCL1 gene. The TCL1 gene was abnormally expressed (A) in patient leukemic cells (P), but not in normal blood cells (N); C, β-actin expression; M, marker (100 bp ladder). Partial sequencing chromatogram (B) of RT-PCR products from the patient and NCBI BLAST search show the identical results for the T-cell receptor alpha delta locus (accession No. 000662.1) that juxtapose TCL1 gene. The TCL1 gene maps at chromosome 14q32.1 and is activated in T cell leukemia and lymphomas by either chromosome translocations or inversions that juxtapose the TCL1 gene to the alpha/delta or the beta locus of the T cell receptor.
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