Journal List > J Korean Assoc Oral Maxillofac Surg > v.37(6) > 1032508

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Chung, Kim, Hwang, Zhang, and Cha: An orthotopic nude mouse model of tongue carcinoma
Original Article

J Korean Assoc Oral Maxillofac Surg 2011;37(6):490-495.

Published online: 10 January 2011

DOI: https://doi.org/10.5125/jkaoms.2011.37.6.490

An orthotopic nude mouse model of tongue carcinoma

Jae-Seung Chung1, Somi Kim1, Young-Sun Hwang2, Xianlan Zhang2, In-Ho Cha1, 2

1Department of Oral and Maxillofacial Surgery, Yonsei University, Seoul, Korea

2Oral Cancer Research Institute, College of Dentistry, Yonsei University, Seoul, Korea

In-Ho Cha Department of Oral and Maxillofacial Surgery College of Dentistry, Yonsei University 50 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Korea TEL: +82-2-2228-8750 FAX: +82-2-2227-8022 E-mail: cha8764@yuhs.ac

Received 26 September 2011       Revised 1 November 2011       Accepted 1 November 2011

Copyright © 2011 Korean Association of Oral and Maxillofacial Surgeons

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Introduction

Development of carcinoma on oral tongue may cause bilateral cervical lymph node metastasis, rapid invasion and growth of the cancer cells due to rich blood supply in muscle tissues. It is not only difficult to develop an animal experimental model, but also to proceed follow-up research after the development of such model as the induction of cancer lead to difficulty in taking nutrition for the experimental animals that often causes early death.

Materials and Methods

IIn this study, author have transplanted YD-10Bmod cells into nude mouse oral tongues with different cells number (5×104, 5×105, 5×106 cells/mouse) and observed the development aspect of oral tongue cancers.

Results

The cancer developed from orthotopic transplantation of YD-10Bmod cells into nude mouse oral tongue show invasion and central necrosis of the tumor, similar to the cancers developed human oral tongue cancer. The difference in tumor size and the time of central necrosis development depending on the number of transplanted tumor cells shows the feasibility of extending the survival period of the nude mouse by limiting the transplanted tumor cells to <5×104 cells/mouse or under per nude mouse.

Conclusion

This nude mouse model could be used effectively in developing effective chemotheray agent and establishing an animal experimental model that can be used to study the mechanism of cervical lymph node metastasis of the oral tongue cancer.

Keywords: Tongue, Squamous cell carcinoma, Cell line

References

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Fig. 1.
Tumor formation on lateral border of tongue. Jae-Seung Chung et al: An orthotopic nude mouse model of tongue carcinoma. J Korean Assoc Oral Maxillofac Surg 2011
jkaoms-37-490f1.tif
Fig. 2.
There is no tumor formation by subcutaneous inoculation of media only in control group (3 weeks, H&E staining, ×40). Jae-Seung Chung et al: An orthotopic nude mouse model of tongue carcinoma. J Korean Assoc Oral Maxillofac Surg 2011
jkaoms-37-490f2.tif
Fig. 3.
The histopathologic findings of tumor (arrowheads) of YD-10Bmod cells (5×104 cells/mouse, 3 weeks). The tumor (T) showed invasive growth into surrounding muscle tissue (M) (H&E staining, A: ×40, B: ×200). Jae-Seung Chung et al: An orthotopic nude mouse model of tongue carcinoma. J Korean Assoc Oral Maxillofac Surg 2011
jkaoms-37-490f3.tif
Fig. 4.
The histopathologic findings of tumor of YD-10Bmod cells (5×105 cells/mouse, 3 weeks). The tumor (T) showed invasive growth into surrounding tissue. The tumor cells showed cellular dysplasia and abnormal mitosis (arrowheads) (H&E staining, A: ×40, B: ×200). Jae-Seung Chung et al: An orthotopic nude mouse model of tongue carcinoma. J Korean Assoc Oral Maxillofac Surg 2011
jkaoms-37-490f4.tif
Fig. 5.
The histopathologic findings of tumor of YD-10Bmod cells (5×106 cells/mouse, 3 weeks). The tumor (T) showed invasive growth into surrounding tissue. The tumor showed cellular pleomorphism and central necrosis (N, arrowheads) (H&E staining, A: ×40, B: ×200). Jae-Seung Chung et al: An orthotopic nude mouse model of tongue carcinoma. J Korean Assoc Oral Maxillofac Surg 2011
jkaoms-37-490f5.tif
Fig. 6.
The histopathologic findings of tumor of YD-10Bmod cells (5×106 cells/mouse, 2 weeks). The tumor (T) showed invasive growth into peripheral nerve sheath (N) (H&E staining, ×200). Jae-Seung Chung et al: An orthotopic nude mouse model of tongue carcinoma. J Korean Assoc Oral Maxillofac Surg 2011
jkaoms-37-490f6.tif
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