Journal List > J Korean Assoc Oral Maxillofac Surg > v.37(6) > 1032508

J Korean Assoc Oral Maxillofac Surg. 2011 Dec;37(6):490-495. Korean.
Published online December 27, 2011.  https://doi.org/10.5125/jkaoms.2011.37.6.490
Copyright © 2011 by The Korean Association of Oral and Maxillofacial Surgeons
An orthotopic nude mouse model of tongue carcinoma
Jae-Seung Chung,1 Somi Kim,1 Young-Sun Hwang,2 Xianlan Zhang,2 and In-Ho Cha1,2
1Department of Oral and Maxillofacial Surgery, College of Dentistry, Yonsei University, Seoul, Korea.
2Oral Cancer Research Institute, College of Dentistry, Yonsei University, Seoul, Korea.

Corresponding author: In-Ho Cha. Department of Oral and Maxillofacial Surgery, College of Dentistry, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Korea. TEL: +82-2-2228-8750, FAX: +82-2-2227-8022, Email: cha8764@yuhs.ac
Received September 26, 2011; Revised November 01, 2011; Accepted November 01, 2011.

Abstract

Introduction

Development of carcinoma on oral tongue may cause bilateral cervical lymph node metastasis, rapid invasion and growth of the cancer cells due to rich blood supply in muscle tissues. It is not only difficult to develop an animal experimental model, but also to proceed follow-up research after the development of such model as the induction of cancer lead to difficulty in taking nutrition for the experimental animals that often causes early death.

Materials and Methods

IIn this study, author have transplanted YD-10Bmod cells into nude mouse oral tongues with different cells number (5×104, 5×105, 5×106 cells/mouse) and observed the development aspect of oral tongue cancers.

Results

The cancer developed from orthotopic transplantation of YD-10Bmod cells into nude mouse oral tongue show invasion and central necrosis of the tumor, similar to the cancers developed human oral tongue cancer. The difference in tumor size and the time of central necrosis development depending on the number of transplanted tumor cells shows the feasibility of extending the survival period of the nude mouse by limiting the transplanted tumor cells to <5×104 cells/mouse or under per nude mouse.

Conclusion

This nude mouse model could be used effectively in developing effective chemotheray agent and establishing an animal experimental model that can be used to study the mechanism of cervical lymph node metastasis of the oral tongue cancer.

Keywords: Tongue; Squamous cell carcinoma; Cell line

Figures


Fig. 1
Tumor formation on lateral border of tongue.
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Fig. 2
There is no tumor formation by subcutaneous inoculation of media only in control group (3 weeks, H&E staining, ×40).
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Fig. 3
The histopathologic findings of tumor (arrowheads) of YD-10Bmod cells (5×104 cells/mouse, 3 weeks). The tumor (T) showed invasive growth into surrounding muscle tissue (M) (H&E staining, A: ×40, B: ×200).
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Fig. 4
The histopathologic findings of tumor of YD-10Bmod cells (5×105 cells/mouse, 3 weeks). The tumor (T) showed invasive growth into surrounding tissue. The tumor cells showed cellular dysplasia and abnormal mitosis (arrowheads) (H&E staining, A: ×40, B: ×200).
Click for larger image


Fig. 5
The histopathologic findings of tumor of YD-10Bmod cells (5×106 cells/mouse, 3 weeks). The tumor (T) showed invasive growth into surrounding tissue. The tumor showed cellular pleomorphism and central necrosis (N, arrowheads) (H&E staining, A: ×40, B: ×200).
Click for larger image


Fig. 6
The histopathologic findings of tumor of YD-10Bmod cells (5×106 cells/mouse, 2 weeks). The tumor (T) showed invasive growth into peripheral nerve sheath (N) (H&E staining, ×200).
Click for larger image

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