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Abstract
Purpose
Calcium phosphate cement (CPC) is one of many useful materials for restoring tooth defects, periodontium and maxillofacial area. Chitosan is a biodegradable material that has been shown to promote the growth and differentiation of osteoblasts in culture. This study examined the interaction between odontoblasts and bio-calcium phosphate cement reinforced with chitosan.
Materials and Methods
5×103 odontoblastic cells were seeded into each well. Various concentrations of bio-calcium phosphate cement reinforced with chitosan (10, 20, 50, 100, 200, 500 μ g/ml, 1, 2, 4 mg/ml) were diluted and added to the wells. The well was incubated for 24 h, 48 h and 72 h. After incubation, the number of cells was assessed to determine the cell viability. A cytokinesis-block micronucleus assay and chromosomal aberration test were carried out to estimate the extent of chromosomal abnormalities. Microscopic photographs and RT-PCR were performed to examine the adhesion potential of bio-calcium phosphate cement reinforced with chitosan.
Results
Bio-CPC-reinforced chitosan did not show significant cytotoxicity. The number of damaged chromosomes in the cells treated with Bio-CPC-reinforced chitosan was similar to that in the control cells. There was no significant increase in the number of chromosomal aberrations in the Bio-CPC reinforced chitosan exposed cells. Microscopic photographs and RT-PCR confirmed the adhesive potential of bio-CPC reinforced chitosan to odontoblasts.
Conclusion
Bio-CPC-reinforced chitosan did not affect the odontoblastic cell viability, and had no significant cytotoxic effect. Bio-CPC-reinforced chitosan showed adhesive potential to odontoblasts. These results are expected form the basis of future studies on the effectiveness of dental restorative materials in Bio-CPC reinforced with chitosan.
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Fig. 1.
The effects of cements on cell viability. Byung-Do Chun et al: Interaction between odontoblast and bio-calcium phosphate cement reinforced with chitosan. J Korean Assoc Oral Maxillofac Surg 2011
Fig. 2.
Cytokinesis-block micronucleus assay. Byung-Do Chun et al: Interaction between odontoblast and bio-calcium phosphate cement reinforced with chitosan. J Korean Assoc Oral Maxillofac Surg 2011
Fig. 4.
Microscopic photography of the cell adhesion. A: coated plate, B: non-coated plate, C: non-coated plate with CPC-ch. Byung-Do Chun et al: Interaction between odontoblast and bio-calcium phosphate cement reinforced with chitosan. J Korean Assoc Oral Maxillofac Surg 2011
Fig. 5.
The expression of mRNAs in MDPC-23 cells. Byung-Do Chun et al: Interaction between odontoblast and bio-calcium phosphate cement reinforced with chitosan. J Korean Assoc Oral Maxillofac Surg 2011
Table 1.
Reverse transcriptase-polymerase chain reaction (RT-PCR) primers sequence
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