Bone regeneration of the fluoridated hydroxyapatite and the bio-glass in the rabbit cranium defect model

Se-Jin Han; Kyung-Wook Kim; Hyo-Joon Ahn; Se-Jin Han; Kyung-Wook Kim

doi:10.5125/jkaoms.2011.37.5.380

Journal List > J Korean Assoc Oral Maxillofac Surg > v.37(5) > 1032492

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Han, Kim, Ahn, Han, and Kim: Bone regeneration of the fluoridated hydroxyapatite and the bio-glass in the rabbit cranium defect model

Original Article

J Korean Assoc Oral Maxillofac Surg 2011;37(5):380-385.

Published online: 10 January 2011

DOI: https://doi.org/10.5125/jkaoms.2011.37.5.380

Bone regeneration of the fluoridated hydroxyapatite and the bio-glass in the rabbit cranium defect model

Se-Jin Han, Kyung-Wook Kim, Hyo-Joon Ahn, Se-Jin Han, Kyung-Wook Kim

Department of Oral and Maxillofacial Surgery, School of Dentistry, Dankook University, Cheonan, Korea

Kyung-Wook Kim Department of Oral and Maxillofacial Surgery, Dental Hospital, Dankook University San 7-1, Sinbu-dong, Cheonan, 330-716, Korea TEL: +82-41-550-1994 FAX: +82-41-551-8988 E-mail: kkwoms@dku.edu

Received 6 May 2011 Revised 30 September 2011 Accepted 29 September 2011

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Introduction

Hydroxyapatite (Ca10(PO₄)6(OH)2, HA) is the main inorganic phase of human hard tissue that is used widely as the repair material for bones. When HA is applied to a bony defect, however, it can be encapsulated with fibrous tissue and float in the implanted area due to a lack of consolidation. Bioceramics as allogenic graft materials are added to HA to improve the rate and bone healing capacity. Fluoridated hydroxyapatite (Ca10(PO₄)6(OH, F)2, FHA), where F- partially replaces the OH- in hydroxyapatite, is considered a good alternative material for bone repair owing to its solubility and biocompatibility.

Materials and Methods

This study was designed to determine the bone healing capacity of FHA newly produced as a nanoscale fiber in the laboratory. HA and FHA with bioglass was implanted in a rabbit cranium defect and the specimen was analysed histologically.

Results

1. At 4 weeks, fibrous connective tissue and little bone formation was observed around the materials of the experimental group I implanted HA and bioglass. Newly formed bone was observed around the materials in the experimental group II implanted FHA and bioglass. 2. At 8 weeks, the amount of newly formed and matured bone was higher in experimental group II than in experimental group I and the control group.

Conclusion

These results suggest that FHA and bioglass is a relatively favorable bone substitute with biocompatibility and better bone healing capacity than pure HA and bioglass.

Keywords: Durapatite, Fluor-hydroxylapatite, Bioglass, Bone Substitutes

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Fig. 1.

H&E staining findings of control group, 4 weeks (×10). Hyo-Joon Ahn et al: Bone regeneration of the fluoridated hydroxyapatite and the bioglass in the rabbit cranium defect model. J Korean Assoc Oral Maxillofac Surg 2011

Fig. 2.

H&E staining findings of experimental group I, 4 weeks (×10). Hyo-Joon Ahn et al: Bone regeneration of the fluoridated hydroxyapatite and the bioglass in the rabbit cranium defect model. J Korean Assoc Oral Maxillofac Surg 2011

Fig. 3.

H&E staining findings of experimental group I, 8 weeks (×10). Hyo-Joon Ahn et al: Bone regeneration of the fluoridated hydroxyapatite and the bioglass in the rabbit cranium defect model. J Korean Assoc Oral Maxillofac Surg 2011

Fig. 4.

H&E staining findings of experimental group II, 4 weeks (×10). Hyo-Joon Ahn et al: Bone regeneration of the fluoridated hydroxyapatite and the bioglass in the rabbit cranium defect model. J Korean Assoc Oral Maxillofac Surg 2011

Fig. 5.

H&E staining findings of experimental group II, 8 weeks (×10). Hyo-Joon Ahn et al: Bone regeneration of the fluoridated hydroxyapatite and the bioglass in the rabbit cranium defect model. J Korean Assoc Oral Maxillofac Surg 2011

Fig. 6.

MT staining findings of control group, 4 weeks (×10). Hyo-Joon Ahn et al: Bone regeneration of the fluoridated hydroxyapatite and the bioglass in the rabbit cranium defect model. J Korean Assoc Oral Maxillofac Surg 2011

Fig. 7.

MT staining findings of control group, 8 weeks (×5). Hyo-Joon Ahn et al: Bone regeneration of the fluoridated hydroxyapatite and the bioglass in the rabbit cranium defect model. J Korean Assoc Oral Maxillofac Surg 2011

Fig. 8.

MT staining findings of experimental group I, 4 weeks (×10). Hyo-Joon Ahn et al: Bone regeneration of the fluoridated hydroxyapatite and the bioglass in the rabbit cranium defect model. J Korean Assoc Oral Maxillofac Surg 2011

Fig. 9.

MT staining findings of experimental group I, 8 weeks (×5). Hyo-Joon Ahn et al: Bone regeneration of the fluoridated hydroxyapatite and the bioglass in the rabbit cranium defect model. J Korean Assoc Oral Maxillofac Surg 2011

Fig. 10.

MT staining findings of experimental group II, 4 weeks (×10). Hyo-Joon Ahn et al: Bone regeneration of the fluoridated hydroxyapatite and the bioglass in the rabbit cranium defect model. J Korean Assoc Oral Maxillofac Surg 2011

Fig. 11.

MT staining findings of experimental group II, 8 weeks (×5). Hyo-Joon Ahn et al: Bone regeneration of the fluoridated hydroxyapatite and the bioglass in the rabbit cranium defect model. J Korean Assoc Oral Maxillofac Surg 2011

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