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Abstract
Introduction
The purpose of this study is to evaluate the effect of platelet rich plasma in bone formation and osseointegration of implant installed in the bone marrow space.
Materials and Methods
Five beagle dogs were used as experimental animals. Two implants were installed on each tibia in the dogs. Implants of the control group were installed with no additional graft. Those of the experimental group 1 were installed with autogenous bone graft from the ilium autogenous bone. Platelet rich plasma (PRP) alone was used in experimental group 2 and the mixture of PRP and autogenous bone was used in experimental group 3. The animals were sacrificed at 8 weeks after implantation. The parts of tibia containing implant were harvested and radiographs were taken for radiographic examination. The specimens were prepared for histological examination and histomorphometric analysis of implant-bone contact ratios.
Results
1. All implants showed sufficient osseointegration in the cortical bone radiographically and histologically, but osseointergration in the marrow space was not satisfactory. 2. Histomorphometrically, the implant-bone contact ratios in the bone marrow was sequentially high in the experimental group 3 (autogenous bone + PRP group), group 1 (autogenous bone group), group 2 (PRP group), and control group (non-additive). 3. It was verified that there was statistical significance between two experimental groups (group 1 and 3) and the other groups (group 2 and control group). (P<0.005) 4. However, there was no statistical significance between group 3 and group 1, also group 2 and control group respectively.
References
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Fig. 1.
Radiograph and photomicrographs of control group. A. The tibia shows thick dense cortical bone and marrow space. B. The implant installed in tibia is observed. (H&E, original magnificaion x1) C. Cortical bone area. The implant threads are filled with dense bone. (H&E, original magnificaion x40) D. Bone marrow space. The implant surface in marrow space shows contact with some cancellous bone. (H&E, original magnificaion x40) (H&E: hematoxylin eosin)
Fig. 2.
Radiograph and photomicrographs of experimental group 1. A. The tibia shows thick dense cortical bone and marrow space. B. The implant installed in tibia is observed. (H&E, original magnificaion x1) C. Cortical bone area. The implant threads in cortical bone. (H&E, original magnificaion x40) D. Bone marrow space. More dense bone contacts with the implant surface. (H&E, original magnificaion x40) (H&E: hematoxylin eosin)
Fig. 3.
Radiograph and photomicrographs of experimental group 2. A. The tibia shows thick dense cortical bone and marrow space. B. The implant installed in tibia is observed. (H&E, original magnificaion x1) C. Cortical bone area. The implant threads in cortical bone. (H&E, original magnificaion x40) D. Bone marrow space. The implant surface in marrow space shows contact with some cancellous bone. (H&E, original magnificaion x40) (H&E: hematoxylin eosin)
Fig. 4.
Radiograph and photomicrographs of experimental group 3. A. The tibia shows thick dense cortical bone and marrow space. B. The implant installed in tibia is observed. (H&E, original magnificaion x1) C. Cortical bone area. The implant threads in cortical bone. (H&E, original magnificaion x40) D. Bone marrow space. The implant surface is contacted with cancellous bone and more bone was observed in the marrow space. (H&E, original magnificaion x40) (H&E: hematoxylin eosin)
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