Evaluation of bone formation by recombinant human BMP-2 and rapid prototype titanium cap in rabbit calvaria using micro computed tomography

Man-Seung Han; Seunggon Jung; Bang-Sin Kim; Ji-Woong Yang; Min-Suk Kook; Hong-Ju Park; Sun-Youl Ryu; Hee-Kyun Oh

doi:10.5125/jkaoms.2010.36.6.466

Journal List > J Korean Assoc Oral Maxillofac Surg > v.36(6) > 1032431

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Han, Jung, Kim, Yang, Kook, Park, Ryu, and Oh: Evaluation of bone formation by recombinant human BMP-2 and rapid prototype titanium cap in rabbit calvaria using micro computed tomography

Original Article

J Korean Assoc Oral Maxillofac Surg 2010;36(6):466-472.

Published online: 7 January 2010

DOI: https://doi.org/10.5125/jkaoms.2010.36.6.466

Evaluation of bone formation by recombinant human BMP-2 and rapid prototype titanium cap in rabbit calvaria using micro computed tomography

Man-Seung Han, Seunggon Jung, Bang-Sin Kim, Ji-Woong Yang, Min-Suk Kook, Hong-Ju Park, Sun-Youl Ryu, Hee-Kyun Oh

Department of Oral and Maxillofacial Surgery, 2nd stage of Brain Korea 21 (BK21), School of Dentistry, Chonnam National University, Dental Science Research Institute, Chonnam National University, Gwangju, Korea

Hee-Kyun Oh Department of Oral and Maxillofacial Surgery, School of Dentistry, Chonnam National University 77 Yongbong-ro, Buk-gu, Gwangju, 500-757, Korea TEL: +82-62-530-5618 FAX: +82-62-530-5619 E-mail: hkoh@chonnam.ac.kr

Received 29 July 201017 November 2010 Accepted 14 December 2010

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Introduction

This study examined the effect of recombinant human bone morphogenetic protein (rhBMP)-2 and β -tricalcium phosphate (β -TCP) on new bone formation in a rabbit calvarium using a rapid prototype titanium cap (RP Ti cap).

Materials and Methods

Eight New Zealand white rabbits were used in this study. Hemispherical RP Ti caps (10 mm in diameter) were implanted subperiosteally on the rabbit calvaria. β -TCP was filled in the RP Ti cap in the control group, and rhBMP-2 soaked β -TCP was used in experimental group. The rabbits were sacrificed 2 and 4 weeks after the operation. The volume and pattern of newly formed bone was analyzed by micro computed tomography (CT).

Results

Macroscopically, there were no abnormal findings in any of the animals. The micro CT images revealed new bone from the calvaria that expanded gradually toward the top of the titanium cap, particularly along the inner surface of the titanium cap in the experimental group at 4 weeks after grafting. There was no significant difference in new bone volume ratio between the control and experimental groups at 2 weeks after grafting. There was a statistically significant difference in the new bone volume ratio between the experimental (14.1±1.8 %) and control (7.2±1.5 %) groups at 4 weeks after grafting (P<0.01).

Conclusion

The RP Ti cap can effectively guide new bone formation and rhBMP-2 can induce the new bone formation.

Keywords: Titanium cap, Bone morphogenetic protein 2, Micro computed tomography

REFERENCES

1. Dahlin C. Scientific background of guided bone regeneration. Buser D, Dahlin C, Schenk RK, editors. Guided bone regeneration in implant dentistry. Chicago: Quintessence Publishing Co. Inc.;1994. p. 31–48.

2. Kostopoulos L, Karring T. Augmentation of the rat mandible using guided tissue regeneration. Clin Oral Implants Res. 1994; 5:75–82.

3. Lundgren D, Lundgren AK, Sennerby L, Nyman S. Augmentation of intramembraneous bone beyond the skeletal envelope using an occlusive titanium barrier. An experimental study in the rabbit. Clin Oral Implants Res. 1995; 6:67–72.

4. Yamada Y, Nanba K, Ito K. Effects of occlusiveness of a titanium cap on bone generation beyond the skeletal envelope in the rabbit calvarium. Clin Oral Implants Res. 2003; 14:455–63.

5. van Steenberghe D, Johansson C, Quirynen M, Molly L, Albrektsson T, et al. Bone augmentation by means of a stiff occlusive titanium barrier. Clin Oral Implants Res. 2003; 14:63–71.

6. Nishida T, Yamada Y, Murai M, Shimizu Y, Oshikawa M, Ito K. Effects of bioactive glass on bone augmentation within a titanium cap in rabbit parietal bone. J Periodontol. 2006; 77:983–9.

7. Min S, Sato S, Murai M, Okuno K, Fujisaki Y, Yamada Y, et al. Effects of marrow penetration on bone augmentation within a titanium cap in rabbit calvarium. J Periodontol. 2007; 78:1978–84.

8. Tamura T, Fukase Y, Goke E, Yamada Y, Sato S, Nishiyama M, et al. Three-dimensional evaluation for augmented bone using guided bone regeneration. J Periodontal Res. 2005; 40:269–76.

9. Yamada Y, Tamura T, Hariu K, Asano Y, Sato S, Ito K. Angiogenesis in newly augmented bone observed in rabbit calvarium using a titanium cap. Clin Oral Implants Res. 2008; 19:1003–9.

10. Min S, Sato S, Saito M, Ebihara H, Arai Y, Ito K. Micro-computerized tomography analysis: dynamics of bone augmentation within a titanium cap in rabbit calvarium. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008; 106:892–5.

11. Molly L, Quirynen M, Michiels K, van Steenberghe D. Comparison between jaw bone augmentation by means of a stiff occlusive titanium membrane or an autologous hip graft: a retrospective clinical assessment. Clin Oral Implants Res. 2006; 17:481–7.

12. Schmid J, Wallkamm B, Ha ¨mmerle CH, Gogolewski S, Lang NP. The significance of angiogenesis in guided bone regeneration. A case report of a rabbit experiment. Clin Oral Implants Res. 1997; 8:244–8.

13. Tamura K, Sato S, Kishida M, Asano S, Murai M, Ito K. The use of porous beta-tricalcium phosphate blocks with platelet-rich plasma as an onlay bone graft biomaterial. J Periodontol. 2007; 78:315–21.

14. Ogose A, Hotta T, Hatano H, Kawashima H, Tokunaga K, Endo N, et al. Histological examination of beta-tricalcium phosphate graft in human femur. J Biomed Mater Res. 2002; 63:601–4.

15. Urist MR. Bone formation by autoinduction. Science. 1965; 150:893–9.

16. Asahina I, Sampath TK, Hauschka PV. Human osteogenic protein-1 induces chondroblastic, osteoblastic, and/or adipocytic differentiation of clonal murine target cells. Exp Cell Res. 1996; 222:38–47.

17. Matsushita N, Terai H, Okada T, Nozaki K, Inoue H, Miyamoto S, et al. A new bone-inducing biodegradable porous beta-tricalcium phosphate. J Biomed Mater Res A. 2004; 70:450–8.

18. Alam I, Asahina I, Ohmamiuda K, Enomoto S. Comparative study of biphasic calcium phosphate ceramics impregnated with rhBMP-2 as bone substitutes. J Biomed Mater Res. 2001; 54:129–38.

19. Jingushi S, Urabe K, Okazaki K, Hirata G, Sakai A, Ikenoue T, et al. Intramuscular bone induction by human recombinant bone morphogenetic protein-2 with beta-tricalcium phosphate as a carrier: in vivo bone banking for muscle-pedicle autograft. J Orthop Sci. 2002; 7:490–4.

20. Laffargue P, Hildebrand HF, Rtaimate M, Frayssinet P, Amoureux JP, Marchandise X. Evaluation of human recombinant bone morphogenetic protein-2-loaded tricalcium phosphate implants in rabbits’ bone defects. Bone. 1999; 25(2 Suppl):55S–58S.

21. Urist MR, Nilsson O, Rasmussen J, Hirota W, Lovell T, Schmalzreid T, et al. Bone regeneration under the influence of a bone morphogenetic protein (BMP) beta tricalcium phosphate (TCP) composite in skull trephine defects in dogs. Clin Orthop Relat Res. 1987; (214):295–304.

22. Mu ¨ller R, van Campenhout H, van Damme B, van Der Perre G, Dequeker J, Hildebrand T, et al. Morphometric analysis of human bone biopsies: a quantitative structural comparison of histological sections and micro-computed tomography. Bone. 1998; 23:59–66.

23. Fajardo RJ, Mu ¨ller R. Three-dimensional analysis of nonhuman primate trabecular architecture using micro-computed tomography. Am J Phys Anthropol. 2001; 115:327–36.

24. Balto K, Mu ¨ller R, Carrington DC, Dobeck J, Stashenko P. Quantification of periapical bone destruction in mice by micro-computed tomography. J Dent Res. 2000; 79:35–40.

Fig. 1.

Rapid prototype titanium cap is 5 mm in height, 10 mm in diameter and 1 mm in thickness with rough surface.

Fig. 2.

Progress in calculation of new bone formation.

Fig. 3.

Micro CT images of 2 weeks after grafting in control group. (CT: computed tomography)

Fig. 4.

Micro CT images of 2 weeks after grafting in experimental group. (CT: computed tomography)

Fig. 5.

Micro CT images of 4 weeks after grafting in control group. (CT: computed tomography)

Fig. 6.

Micro CT images of 4 weeks after grafting in experimental group. (CT: computed tomography)

Fig. 7.

New bone volume ratio (%) at 2 and 4 weeks after grafting.

Table 1.

Volume ratio (%) of mineralized bone, β-TCP and newly generated bone under RP titanium cap (unit: %)

Groups	2 weeks			4 weeks
Groups	PBV	TCPV	NBV	PBV	TCPV	NBV
Control	26.2	19.3	6.9	23.9	16.7	7.2^∗
Experimental	28.4	19.9	8.5^∗∗	31.6^∗∗	17.5^∗∗	14.1^∗∗

^∗ (P<0.01

^∗∗ P<0.01 (ANOVA), TCP: tricalcium phosphate, RP: rapid prototype, BV: bone volume, TV: tissue volume, PBV: percent bone volume (BV/TV), TCPV: percent β -TCP volume/TV, NBV: new bone volume (PBV-TCPV))

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